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Literature DB >> 9421528

Electrospray ionization mass spectrometric characterization of photocrosslinked DNA-EcoRI DNA methyltransferase complexes.

Abstract

We describe a novel strategy combining photocrosslinking and HPLC-based electrospray ionization mass spectrometry to identify UV crosslinked DNA-protein complexes. Eco RI DNA methyltransferase modifies the second adenine within the recognition sequence GAATTC. Substitution of 5-iodouracil for the thymine adjacent to the target base (GAATTC) does not detectably alter the DNA-protein complex. Irradiation of the 5-iodouracil-substituted DNA-protein complex at various wavelengths was optimized, with a crosslinking yield >60% at 313 nm after 1 min. No protein degradation was observed under these conditions. The crosslinked DNA-protein complex was further analyzed by electrospray ionization mass spectrometry. The total mass is consistent with irradiation-dependent covalent bond formation between one strand of DNA and the protein. These preliminary results support the possibility of identifying picomole quantities of crosslinked peptides by similar strategies.

Entities: Chemical

Mesh：

Substances：

Year: 1998 PMID： 9421528 PMCID： PMC147290 DOI： 10.1093/nar/26.2.645

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

26 in total

1. Sequence-specific recognition of cytosine C5 and adenine N6 DNA methyltransferases requires different deformations of DNA.

Authors: R A Garcia; C J Bustamante; N O Reich
Journal: Proc Natl Acad Sci U S A Date: 1996-07-23 Impact factor: 11.205

2. Facilitated diffusion of the EcoRI DNA methyltransferase is described by a novel mechanism.

Authors: M A Surby; N O Reich
Journal: Biochemistry Date: 1996-02-20 Impact factor: 3.162

3. Characterization of peptide-oligonucleotide heteroconjugates by mass spectrometry.

Authors: O N Jensen; S Kulkarni; J V Aldrich; D F Barofsky
Journal: Nucleic Acids Res Date: 1996-10-01 Impact factor: 16.971

4. Preparation of oligoribonucleotides containing 4-thiouridine using Fpmp chemistry. Photo-crosslinking to RNA binding proteins using 350 nm irradiation.

Authors: A McGregor; M V Rao; G Duckworth; P G Stockley; B A Connolly
Journal: Nucleic Acids Res Date: 1996-08-15 Impact factor: 16.971

5. Application of electrospray and fast atom bombardment mass spectrometry to the identification of post-translational and other chemical modifications of proteins and peptides.

Authors: M Kouach; D Belaïche; M Jaquinod; M Couppez; D Kmiecik; G Ricart; A Van Dorsselaer; P Sautière; G Briand
Journal: Biol Mass Spectrom Date: 1994-05

6. An RNA-protein contact determined by 5-bromouridine substitution, photocrosslinking and sequencing.

Authors: M C Willis; K A LeCuyer; K M Meisenheimer; O C Uhlenbeck; T H Koch
Journal: Nucleic Acids Res Date: 1994-11-25 Impact factor: 16.971

7. Three-dimensional structure of the adenine-specific DNA methyltransferase M.Taq I in complex with the cofactor S-adenosylmethionine.

Authors: J Labahn; J Granzin; G Schluckebier; D P Robinson; W E Jack; I Schildkraut; W Saenger
Journal: Proc Natl Acad Sci U S A Date: 1994-11-08 Impact factor: 11.205

8. High yield photocrosslinking of a 5-iodocytidine (IC) substituted RNA to its associated protein.

Authors: K M Meisenheimer; P L Meisenheimer; M C Willis; T H Koch
Journal: Nucleic Acids Res Date: 1996-03-01 Impact factor: 16.971

9. Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence.

Authors: A Chen; L M Powell; D T Dryden; N E Murray; T Brown
Journal: Nucleic Acids Res Date: 1995-04-11 Impact factor: 16.971

10. Crystal structure of a suicidal DNA repair protein: the Ada O6-methylguanine-DNA methyltransferase from E. coli.

Authors: M H Moore; J M Gulbis; E J Dodson; B Demple; P C Moody
Journal: EMBO J Date: 1994-04-01 Impact factor: 11.598

8 in total

1. Characterization of a cross-linked DNA-endonuclease VIII repair complex by electrospray ionization mass spectrometry.

Authors: R A Rieger; M M McTigue; J H Kycia; S E Gerchman; A P Grollman; C R Iden
Journal: J Am Soc Mass Spectrom Date: 2000-06 Impact factor: 3.109

2. Mass spectral characterization of a protein-nucleic acid photocrosslink.

Authors: M C Golden; K A Resing; B D Collins; M C Willis; T H Koch
Journal: Protein Sci Date: 1999-12 Impact factor: 6.725

3. Nested Arg-specific bifunctional crosslinkers for MS-based structural analysis of proteins and protein assemblies.

Authors: Qingrong Zhang; Elizabeth Crosland; Daniele Fabris
Journal: Anal Chim Acta Date: 2008-06-05 Impact factor: 6.558

Review 4. Bacteriophage T4 genome.

Authors: Eric S Miller; Elizabeth Kutter; Gisela Mosig; Fumio Arisaka; Takashi Kunisawa; Wolfgang Rüger
Journal: Microbiol Mol Biol Rev Date: 2003-03 Impact factor: 11.056

5. Identification of a base-specific contact between the restriction endonuclease SsoII and its recognition sequence by photocross-linking.

Authors: E A Kubareva; H Thole; A S Karyagina; T S Oretskaya; A Pingoud; V Pingoud
Journal: Nucleic Acids Res Date: 2000-03-01 Impact factor: 16.971