Literature DB >> 6327280

Recombinant retroviral DNA yielding high expression of hepatitis B surface antigen.

C Stratowa, J Doehmer, Y Wang, P H Hofschneider.   

Abstract

A genomic fragment of hepatitis B virus encoding the surface antigen (HBsAg) was inserted into the proviral genome of Moloney mouse sarcoma virus (MSV), obtained from the mouse cell line G8 -124. The recombinant DNA was introduced into NIH 3T3 mouse fibroblasts. Cells, morphologically transformed by the oncogene of MSV (v-mosM) were selected, established as cell lines and tested for expression of HBsAg. An expression level of up to 4.5 micrograms/10(7) cells/day was detected.

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Year:  1982        PMID： 6327280      PMCID： PMC553253          DOI： 10.1002/j.1460-2075.1982.tb01357.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  29 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

3.  Preparative and analytical purification of DNA from agarose.

Authors:  B Vogelstein; D Gillespie
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

4.  Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.

Authors:  F R Blattner; B G Williams; A E Blechl; K Denniston-Thompson; H E Faber; L Furlong; D J Grunwald; D O Kiefer; D D Moore; J W Schumm; E L Sheldon; O Smithies
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

5.  Molecular cloning of unintegrated Moloney mouse sarcoma virus DNA in bacteriophage lambda.

Authors:  I M Verma; M H Lai; R A Bosselman; M A McKennett; H Fan; A Berns
Journal:  Proc Natl Acad Sci U S A       Date:  1980-04       Impact factor: 11.205

6.  Identification and molecular cloning of Moloney mouse sarcoma virus-specific sequences from uninfected mouse cells.

Authors:  M Jones; R A Bosselman; F A van der Hoorn; A Berns; H Fan; I M Verma
Journal:  Proc Natl Acad Sci U S A       Date:  1980-05       Impact factor: 11.205

7.  Cellular and humoral immunity in guinea pigs to two major polypeptides derived from hepatitis B surface antigen.

Authors:  G A Cabral; F Marciano-Cabral; G A Funk; Y Sanchez; F B Hollinger; J L Melnick; G R Dreesman
Journal:  J Gen Virol       Date:  1978-02       Impact factor: 3.891

8.  Isolation, characterization, and comparison of recombinant DNAs derived from genomes of human hepatitis B virus and woodchuck hepatitis virus.

Authors:  I W Cummings; J K Browne; W A Salser; G V Tyler; R L Snyder; J M Smolec; J Summers
Journal:  Proc Natl Acad Sci U S A       Date:  1980-04       Impact factor: 11.205

9.  An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA.

Authors:  N D Stow; N M Wilkie
Journal:  J Gen Virol       Date:  1976-12       Impact factor: 3.891

10.  Proteins of hepatitis B surface antigen: amino acid compositions of the major polypeptides.

Authors:  J W Shih; J L Gerin
Journal:  J Virol       Date:  1977-03       Impact factor: 5.103
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  9 in total

1.  Promoter activity and expression of sequences from Ti-plasmid stably maintained in mammalian cells.

Authors:  P Zahm; S L Rhim; K Geider
Journal:  Mol Cell Biochem       Date:  1989-10-05       Impact factor: 3.396

2.  Transcription of hepatitis B virus by RNA polymerase II.

Authors:  L B Rall; D N Standring; O Laub; W J Rutter
Journal:  Mol Cell Biol       Date:  1983-10       Impact factor: 4.272

3.  The 89,000-Mr murine cytomegalovirus immediate-early protein activates gene transcription.

Authors:  U H Koszinowski; G M Keil; H Volkmer; M R Fibi; A Ebeling-Keil; K Münch
Journal:  J Virol       Date:  1986-04       Impact factor: 5.103

4.  Expression of hepatitis B antigens with a simian virus 40 vector.

Authors:  H Will; R Cattaneo; E Pfaff; C Kuhn; M Roggendorf; H Schaller
Journal:  J Virol       Date:  1984-05       Impact factor: 5.103

5.  A virus antigen (HBsAg) indicates metastases of human carcinoma cells in the nude mouse.

Authors:  O Marquardt; A Freytag von Loringhoven; R Koshy; P H Hofschneider; K Miller; J Desmyter
Journal:  Med Microbiol Immunol       Date:  1984       Impact factor: 3.402

6.  Synthesis in animal cells of hepatitis B surface antigen particles carrying a receptor for polymerized human serum albumin.

Authors:  M L Michel; P Pontisso; E Sobczak; Y Malpièce; R E Streeck; P Tiollais
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205

Review 7.  RNA-tumorviruses, oncogenes, and their possible role in human carcinogenesis.

Authors:  R Hehlmann; H Schetters; G Kreeb; V Erfle; J Schmidt; A Luz
Journal:  Klin Wochenschr       Date:  1983-12-15

8.  Enhanced production of hepatitis B surface antigen in NIH 3T3 mouse fibroblasts by using extrachromosomally replicating bovine papillomavirus vector.

Authors:  Y Wang; C Stratowa; M Schaefer-Ridder; J Doehmer; P H Hofschneider
Journal:  Mol Cell Biol       Date:  1983-06       Impact factor: 4.272

9.  Co-transcribed 3' host sequences augment expression of integrated hepatitis B virus DNA.

Authors:  A F von Loringhoven; S Koch; P H Hofschneider; R Koshy
Journal:  EMBO J       Date:  1985-01       Impact factor: 11.598
  9 in total

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