| Literature DB >> 6325397 |
P J McNicol, W L Albritton, A R Ronald.
Abstract
The origin of replication of Haemophilus ducreyi and Neisseria gonorrhoeae ampicillin resistance plasmids was located by cloning BamHI restriction fragments into vector plasmid pAT153 and a derivative plasmid, pAT2. Selection was made for plasmid maintenance in a polA mutant. Direction of replication was determined by in vitro replication of plasmid DNA in the presence of radiolabeled deoxynucleotide.Entities:
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Year: 1984 PMID: 6325397 PMCID: PMC215435 DOI: 10.1128/jb.158.1.393-395.1984
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490