Literature DB >> 6323761

Gamma-T4 hybrid bacteriophage carrying the thymidine kinase gene of bacteriophage T4.

A J Mileham, N E Murray, H R Revel.   

Abstract

Among 32 lambda-T4 recombinant phages selected for growth on a thymidylate synthetase-deficient (thyA) host, 2 were shown to carry the T4 thymidine kinase (tk) gene. The lambda-T4tk phages contain two T4 HindIII DNA fragments (2.0 and 1.5 kilobases) that hybridize to restriction fragments of T4 DNA, encompassing the tk locus at 60 kilobases on the T4 map. The T4tk insert compensates for the simultaneous host deficiencies of thymidine kinase and thymidylate synthetase in a thymidine kinase-deficient (tdk) host growing in the presence of fluorodeoxyuridine when provided with thymidine and uridine. The lambda-T4tk hybrid phages specified five polypeptides with Mrs of 22,000 (22K), 21K, 14K, 11K, and 9K.

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Year:  1984        PMID： 6323761      PMCID： PMC255686     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  15 in total

1.  THYMIDINE-REQUIRING MUTANTS OF PHAGE T4.

Authors:  E H SIMON; I TESSMAN
Journal:  Proc Natl Acad Sci U S A       Date:  1963-09       Impact factor: 11.205

2.  THE MODE OF ACTION OF 5-FLUOROURACIL AND ITS DERIVATIVES.

Authors:  S S Cohen; J G Flaks; H D Barner; M R Loeb; J Lichtenstein
Journal:  Proc Natl Acad Sci U S A       Date:  1958-10-15       Impact factor: 11.205

3.  Temporal appearance of bacteriophage T4-modified valyl tRNA synthetase in Escherichia coli.

Authors:  U R Müller; G L Marchin
Journal:  J Virol       Date:  1975-02       Impact factor: 5.103

4.  Purification and properties of deoxythymidine kinase induced by bacteriophage T4 infection.

Authors:  N Iwatsuki
Journal:  J Biochem       Date:  1977-11       Impact factor: 3.387

5.  A deoxythymidine kinase-deficient mutant of Escherichia coli. I. Isolation and some properties.

Authors:  S Hiraga; K Igarashi; T Yura
Journal:  Biochim Biophys Acta       Date:  1967-08-22

Review 6.  The genome of bacteriophage T4.

Authors:  W B Wood; H R Revel
Journal:  Bacteriol Rev       Date:  1976-12

7.  Physical mapping and cloning of bacteriophage T4 anti-restriction endonuclease gene.

Authors:  K Dharmalingam; H R Revel; E B Goldberg
Journal:  J Bacteriol       Date:  1982-02       Impact factor: 3.490

8.  A restriction map of the bacteriophage T4 genome.

Authors:  P H O'Farrell; E Kutter; M Nakanishi
Journal:  Mol Gen Genet       Date:  1980

9.  Nucleotide sequence of the lysozyme gene of bacteriophage T4. Analysis of mutations involving repeated sequences.

Authors:  J E Owen; D W Schultz; A Taylor; G R Smith
Journal:  J Mol Biol       Date:  1983-04-05       Impact factor: 5.469

10.  Isolation of mutants of bacteriophage T4 unable to induce thymidine kinase activity.

Authors:  K V Chace; D H Hall
Journal:  J Virol       Date:  1973-08       Impact factor: 5.103
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  3 in total

Review 1.  Bacteriophage T4 genome.

Authors:  Eric S Miller; Elizabeth Kutter; Gisela Mosig; Fumio Arisaka; Takashi Kunisawa; Wolfgang Rüger
Journal:  Microbiol Mol Biol Rev       Date:  2003-03       Impact factor: 11.056

2.  Nucleotide sequence and analysis of the 58.3 to 65.5-kb early region of bacteriophage T4.

Authors:  K Valerie; J Stevens; M Lynch; E E Henderson; J K de Riel
Journal:  Nucleic Acids Res       Date:  1986-11-11       Impact factor: 16.971

3.  Physical mapping and complete nucleotide sequence of the denV gene of bacteriophage T4.

Authors:  E H Radany; L Naumovski; J D Love; K A Gutekunst; D H Hall; E C Friedberg
Journal:  J Virol       Date:  1984-12       Impact factor: 5.103
  3 in total

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