Literature DB >> 6323265

Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors.

M M Bagdasarian, E Amann, R Lurz, B Rückert, M Bagdasarian.   

Abstract

A broad-host-range vector, pKT240, containing the structural gene (aph) for aminoglycoside phosphotransferase (APH), without promoter, has been constructed. Insertion of DNA fragments carrying promoters upstream of aph gene into the unique EcoRI site of this vector results in the expression of the aph gene and consequently the resistance of the host cells to streptomycin. The new vector has been used to show that the hybrid trp-lac (tac) promoter and the promoter of the lacIQ gene of Escherichia coli are active in Pseudomonas putida. Derivatives of pKT240 containing tac and lacIQ sequences may be used as wide-host-range expression vectors. Regulated overproduction of APH and catechol 2,3-oxygenase can be obtained with the aid of the new vectors in both E. coli and P. putida.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6323265     DOI： 10.1016/0378-1119(83)90197-x

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  109 in total

1.  The pvc gene cluster of Pseudomonas aeruginosa: role in synthesis of the pyoverdine chromophore and regulation by PtxR and PvdS.

Authors:  A Stintzi; Z Johnson; M Stonehouse; U Ochsner; J M Meyer; M L Vasil; K Poole
Journal:  J Bacteriol       Date:  1999-07       Impact factor: 3.490

2.  Involvement of sigma(S) in starvation-induced transposition of Pseudomonas putida transposon Tn4652.

Authors:  H Ilves; R Hõrak; M Kivisaar
Journal:  J Bacteriol       Date:  2001-09       Impact factor: 3.490

3.  Transcriptional control of the hydrogen cyanide biosynthetic genes hcnABC by the anaerobic regulator ANR and the quorum-sensing regulators LasR and RhlR in Pseudomonas aeruginosa.

Authors:  G Pessi; D Haas
Journal:  J Bacteriol       Date:  2000-12       Impact factor: 3.490

4.  Cloning, nucleotide sequences, and identification of products of the Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity branched-chain amino acid transport system.

Authors:  T Hoshino; K Kose
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

5.  Genetic analysis of the Pseudomonas aeruginosa PAO high-affinity branched-chain amino acid transport system by use of plasmids carrying the bra genes.

Authors:  T Hoshino; K Kose
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

6.  The sequence and function of the recA gene and its protein in Pseudomonas aeruginosa PAO.

Authors:  Y Sano; M Kageyama
Journal:  Mol Gen Genet       Date:  1987-07

7.  In vivo formation of gene fusions in Pseudomonas putida and construction of versatile broad-host-range vectors for direct subcloning of Mu d1 and Mu d2 fusions.

Authors:  V Simon; W Schumann
Journal:  Appl Environ Microbiol       Date:  1987-07       Impact factor: 4.792

8.  Comparison and analysis of the nucleotide sequences of pilin genes from Haemophilus influenzae type b strains Eagan and M43.

Authors:  L J Forney; C F Marrs; S L Bektesh; J R Gilsdorf
Journal:  Infect Immun       Date:  1991-06       Impact factor: 3.441

9.  Nucleotide sequencing and characterization of Pseudomonas putida catR: a positive regulator of the catBC operon is a member of the LysR family.

Authors:  R K Rothmel; T L Aldrich; J E Houghton; W M Coco; L N Ornston; A M Chakrabarty
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

10.  Plasmid introduction in metal-stressed, subsurface-derived microcosms: plasmid fate and community response.

Authors:  Barth F Smets; Jayne B Morrow; Catalina Arango Pinedo
Journal:  Appl Environ Microbiol       Date:  2003-07       Impact factor: 4.792
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.