| Literature DB >> 6323023 |
Abstract
Messenger RNA was prepared from a hybridoma cell line secreting a monoclonal antibody (53FC3) directed against a luminal epitope of a Golgi membrane protein (Mr = 135 kd) found in rodent cells. When this mRNA was microinjected into the cytoplasm of BHK cells, mouse IgG was seen to accumulate in the Golgi complex after 5-6 hr of incubation. No accumulation was seen in 3T3 cells which lack the epitope recognized by 53FC3. When microinjected BHK cells were infected with vesicular stomatitis virus, surface expression of the viral G protein was considerably reduced when compared with neighboring noninjected cells.Entities:
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Year: 1984 PMID： 6323023 PMCID： PMC7133283 DOI： 10.1016/0092-8674(84)90034-5
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582