| Literature DB >> 6319966 |
Abstract
F42lac recombination with lambda plac is normally 20-fold to 50-fold higher than recombination between lambda plac and a chromosomal lac gene. Transductional crosses with lambda plac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42lac.Entities:
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Year: 1984 PMID: 6319966 DOI: 10.1007/bf00330679
Source DB: PubMed Journal: Mol Gen Genet ISSN: 0026-8925