Literature DB >> 6317893

Isolation and partial chemical characterization of a 64,000-dalton glycoprotein of human cytomegalovirus.

B R Clark, J A Zaia, L Balce-Directo, Y P Ting.   

Abstract

A guanidinium chloride extract of [3H]glucosamine- and [35S]methionine-labeled virions plus dense bodies of human cytomegalovirus (Towne) was separated by reverse-phase high-pressure liquid chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the eluate revealed the major peak to be a glycoprotein with a relative mass of 64,000. This glycoprotein (HCMVgp64) was characterized by amino acid analysis and a high-pressure liquid chromatographic map of its tryptic peptides.

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Year:  1984        PMID： 6317893      PMCID： PMC255455     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  10 in total

1.  The morphogenesis of human cytomegalovirus. Isolation and polypeptide characterization of cytomegalovirions and dense bodies.

Authors:  I Sarov; I Abady
Journal:  Virology       Date:  1975-08       Impact factor: 3.616

2.  Antibody to varicella-zoster virus-induced membrane antigen: immunofluorescence assay using monodisperse glutaraldehyde-fixed target cells.

Authors:  J A Zaia; M N Oxman
Journal:  J Infect Dis       Date:  1977-10       Impact factor: 5.226

3.  Viscosity-density gradient for purification of foot-and-mouth disease virus.

Authors:  R Barzilai; L H Lazarus; N Goldblum
Journal:  Arch Gesamte Virusforsch       Date:  1972

4.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

5.  Two-column system for determination of glucosamine, galactosamine, and amino acids on a Beckman 121MB amino acid analyzer: separation of the anomers of glucosamine and galactosamine.

Authors:  U Del Valle; J E Shively
Journal:  Anal Biochem       Date:  1979-07-01       Impact factor: 3.365

6.  Electrophoretic analysis of polypeptides immune precipitated from cytomegalovirus-infected cell extracts by human sera.

Authors:  L Pereira; M Hoffman; N Cremer
Journal:  Infect Immun       Date:  1982-06       Impact factor: 3.441

7.  Monoclonal antibodies to human cytomegalovirus: three surface membrane proteins with unique immunological and electrophoretic properties specify cross-reactive determinants.

Authors:  L Pereira; M Hoffman; D Gallo; N Cremer
Journal:  Infect Immun       Date:  1982-06       Impact factor: 3.441

8.  Human cytomegalovirus: glycoproteins associated with virions and dense bodies.

Authors:  M F Stinski
Journal:  J Virol       Date:  1976-08       Impact factor: 5.103

9.  Analysis of structural polypeptides of purified human cytomegalovirus.

Authors:  K S Kim; V J Sapienza; R I Carp; H M Moon
Journal:  J Virol       Date:  1976-12       Impact factor: 5.103

10.  Cytomegalovirus proteins. I. Polypeptides of virions and dense bodies.

Authors:  M Fiala; R W Honess; D C Heiner; J W Heine; J Murnane; R Wallace; L B Guze
Journal:  J Virol       Date:  1976-07       Impact factor: 5.103
  10 in total
  10 in total

1.  Molecular cloning and analysis of three cDNA clones homologous to human cytomegalovirus RNAs present during late infection.

Authors:  J Martinez; S C St Jeor
Journal:  J Virol       Date:  1986-11       Impact factor: 5.103

2.  Immunofluorescence for detection of antibodies against human cytomegalovirus-induced membrane antigens.

Authors:  J M Middeldorp; J Jongsma; T H The
Journal:  J Clin Microbiol       Date:  1986-09       Impact factor: 5.948

3.  Mapping and expression of a human cytomegalovirus major viral protein.

Authors:  M G Davis; E C Mar; Y M Wu; E S Huang
Journal:  J Virol       Date:  1984-10       Impact factor: 5.103

4.  Primary structure and transcription of the genes coding for the two virion phosphoproteins pp65 and pp71 of human cytomegalovirus.

Authors:  B Rüger; S Klages; B Walla; J Albrecht; B Fleckenstein; P Tomlinson; B Barrell
Journal:  J Virol       Date:  1987-02       Impact factor: 5.103

5.  Isolation by high-performance liquid chromatography and partial characterization of a 57,000-dalton herpes simplex virus type 1 polypeptide.

Authors:  S Welling-Wester; T Popken-Boer; J B Wilterdink; J van Beeumen; G W Welling
Journal:  J Virol       Date:  1985-05       Impact factor: 5.103

6.  Characterization of human monoclonal antibodies directed against the pp65-kD matrix antigen of human cytomegalovirus.

Authors:  M Ohlin; V A Sundqvist; G Gilljam; U Rudén; F O Gombert; B Wahren; C A Borrebaeck
Journal:  Clin Exp Immunol       Date:  1991-06       Impact factor: 4.330

7.  Comparative study with monospecific and monoclonal antibodies against a 65 K human cytomegalovirus protein.

Authors:  K Shimokawa; X Bin; T Furukawa
Journal:  Arch Virol       Date:  1988       Impact factor: 2.574

8.  Procaryotic expression of phosphorylated tegument protein pp65 of human cytomegalovirus and application of recombinant peptides for immunoblot analyses.

Authors:  B Plachter; S Klages; S Hagelmann; W Britt; M P Landini; G Jahn
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

9.  Cloning and physical mapping of a gene fragment coding for a 64-kilodalton major late antigen of human cytomegalovirus.

Authors:  H Pande; S W Baak; A D Riggs; B R Clark; J E Shively; J A Zaia
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

10.  The dominant phosphoprotein pp65 (UL83) of human cytomegalovirus is dispensable for growth in cell culture.

Authors:  S Schmolke; H F Kern; P Drescher; G Jahn; B Plachter
Journal:  J Virol       Date:  1995-10       Impact factor: 5.103
  10 in total

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