Literature DB >> 6317654

Isolation and analysis of aroFo mutants by using an aroF-lac operon fusion.

C S Cobbett, S Morrison, J Pittard.   

Abstract

The lac structural genes were fused to the regulatory region of the aroF-tyrA operon so that the expression of beta-galactosidase was regulated by the tyrR+ gene product. Transducing phage carrying the aroF-lac fusion were isolated, and a lambda aroF-lac lysogen was used to select for aroFo mutants. A plasmid vector was constructed onto which the aroFo mutations were transferred by recombination in vivo.

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Year:  1984        PMID： 6317654      PMCID： PMC215167          DOI： 10.1128/jb.157.1.303-310.1984

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  19 in total

1.  Aromatic biosynthesis. V. Antagonism between shikimic acid and its precursor, 5-dehydroshikimic acid.

Authors:  B D DAVIS
Journal:  J Bacteriol       Date:  1952-11       Impact factor: 3.490

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.

Authors:  N Kleckner; J Roth; D Botstein
Journal:  J Mol Biol       Date:  1977-10-15       Impact factor: 5.469

4.  Regulation of aromatic amino acid transport systems in Escherichia coli K-12.

Authors:  M J Whipp; A J Pittard
Journal:  J Bacteriol       Date:  1977-11       Impact factor: 3.490

5.  Maximizing gene expression on a plasmid using recombination in vitro.

Authors:  K Backman; M Ptashne
Journal:  Cell       Date:  1978-01       Impact factor: 41.582

6.  Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.

Authors:  M J Casadaban
Journal:  J Mol Biol       Date:  1976-07-05       Impact factor: 5.469

7.  Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12.

Authors:  B Ely; J Pittard
Journal:  J Bacteriol       Date:  1979-06       Impact factor: 3.490

8.  Regulation of tyrosine biosynthesis in Escherichia coli K-12: isolation and characterization of operator mutants.

Authors:  I E Mattern; J Pittard
Journal:  J Bacteriol       Date:  1971-07       Impact factor: 3.490

9.  Regulation of tyrosine and phenylalanine biosynthesis in Escherichia coli K-12: properties of the tyrR gene product.

Authors:  H Camakaris; J Pittard
Journal:  J Bacteriol       Date:  1973-09       Impact factor: 3.490

10.  Regulator gene controlling enzymes concerned in tyrosine biosynthesis in Escherichia coli.

Authors:  B J Wallace; J Pittard
Journal:  J Bacteriol       Date:  1969-03       Impact factor: 3.490
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  5 in total

1.  A mutational analysis of the structural basis for transcriptional activation and monomer-monomer interaction in the TyrR system of Escherichia coli K-12.

Authors:  J Cui; R L Somerville
Journal:  J Bacteriol       Date:  1993-03       Impact factor: 3.490

2.  Mutational uncoupling of the transcriptional activation function of the TyrR protein of Escherichia coli K-12 from the repression function.

Authors:  J Cui; R L Somerville
Journal:  J Bacteriol       Date:  1993-01       Impact factor: 3.490

3.  Construction of a tyrP-lac operon fusion strain and its use in the isolation and analysis of mutants derepressed for tyrP expression.

Authors:  P A Kasian; J Pittard
Journal:  J Bacteriol       Date:  1984-10       Impact factor: 3.490

4.  Isolation of an aroF-lac plasmid by recombination in vivo.

Authors:  C S Cobbett; J Pittard
Journal:  J Bacteriol       Date:  1984-01       Impact factor: 3.490

5.  Importance of the position of TYR R boxes for repression and activation of the tyrP and aroF genes in Escherichia coli.

Authors:  A E Andrews; B Dickson; B Lawley; C Cobbett; A J Pittard
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490
  5 in total

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