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Literature DB >> 6316112

A control system which causes alternating turn-off/turn-on of transcription on insertion element IS1.

Abstract

Presence of insertion element IS1 within an operon leads to absence of expression of genes distal to its integration site. This strong polar effect is observed irrespective of the orientation of IS1. Here I report on a mutant of E. coli K12 which allows turn-on of genes located distally to IS1. This turn-on is due to activation of transcription starting within IS1. The activation of transcription is under the control of a regulatory locus (sis1) which acts in trans. The regulatory locus itself changes its state, which leads to an alternating turn-off/turn-on of genes located distal to IS1: The rate of turn-off was observed with a frequency of about 0.3% (per cell and per generation), that of turn-on with a frequency of about 0.015% (per cell and per generation).

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Year: 1983 PMID： 6316112 DOI： 10.1007/bf00327647

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

31 in total

A control system which causes alternating turn-off/turn-on of transcription on insertion element IS1.

Review 1. Regulatory sequences involved in the promotion and termination of RNA transcription.

2. Development of a system useful for studying the formation of unstable alleles of IS2.

3. Relief of polarity in DNA-dependent cell-free synthesis of enzymes of the galactose operon of Escherichia coli.

4. Effect of Rho on transcription of bacterial operons.

5. O0 and strong-polar mutations in the gal operon are insertions.

6. Insertion of IS3 can "turn-on" a silent gene in Escherichia coli.

7. Expression of two proteins from overlapping and oppositely oriented genes on transposable DNA insertion element IS5.

8. Activation of transcription of a yeast gene in E. coli by an IS5 element.

Review 9. Linkage map of Escherichia coli K-12, edition 6.

10. IS2-43 and IS2-44: new alleles of the insertion sequence IS2 which have promoter activity.

1. Location of the bglA gene on the physical map of Escherichia coli.

2. Second-element turn-on of gene expression in an IS1 insertion mutant.

3. Control of phoR-dependent bacterial alkaline phosphatase clonal variation by the phoM region.

4. Insertion element IS5 contains a third gene.

5. High-level ribosomal frameshifting directs the synthesis of IS150 gene products.

6. Activity of Lac repressor anchored to the Escherichia coli inner membrane.