Literature DB >> 6315415

Evidence against a scanning model of RNA splicing.

T Kühne, B Wieringa, J Reiser, C Weissmann.   

Abstract

A cloned rabbit beta-globin gene was modified by introducing a DNA fragment containing the 5' splice region of the large intron upstream or downstream of its natural counterpart. Analogous constructions were carried out with the 3' splice region. The genes were linked to SV40 DNA, transiently expressed in HeLa cells and the transcripts analyzed by S1 mapping. In all cases, the splice site further removed from the intron was utilized to the complete exclusion of its counterpart. This finding argues persuasively against a simple scanning model of RNA splicing, in which the splicing enzyme(s) attaches at the 'donor' spliced region and moves along the intron until it encounters the closest 'acceptor' splice region. A model compatible with the currently known facts is presented.

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Year:  1983        PMID： 6315415      PMCID： PMC555177          DOI： 10.1002/j.1460-2075.1983.tb01492.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  31 in total

1.  Processing and expression of early SV40 mRNA: a role for RNA conformation in splicing.

Authors:  G Khoury; P Gruss; R Dhar; C J Lai
Journal:  Cell       Date:  1979-09       Impact factor: 41.582

2.  Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors:  A J Berk; P A Sharp
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

3.  Cloning specific segments of the mammalian genome: bacteriophage lambda containing mouse globin and surrounding gene sequences.

Authors:  S M Tilghman; D C Tiemeier; F Polsky; M H Edgell; J G Seidman; A Leder; L W Enquist; B Norman; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1977-10       Impact factor: 11.205

4.  A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.

Authors:  L Wieslander
Journal:  Anal Biochem       Date:  1979-10-01       Impact factor: 3.365

5.  Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.

Authors:  R F Weaver; C Weissmann
Journal:  Nucleic Acids Res       Date:  1979-11-10       Impact factor: 16.971

6.  Deletions at intervening sequence splice sites in the alcohol dehydrogenase gene of Drosophila.

Authors:  C Benyajati; A R Place; N Wang; E Pentz; W Sofer
Journal:  Nucleic Acids Res       Date:  1982-11-25       Impact factor: 16.971

7.  Unusual splice sites revealed by mutagenic inactivation of an authentic splice site of the rabbit beta-globin gene.

Authors:  B Wieringa; F Meyer; J Reiser; C Weissmann
Journal:  Nature       Date:  1983-01-06       Impact factor: 49.962

8.  Are snRNPs involved in splicing?

Authors:  M R Lerner; J A Boyle; S M Mount; S L Wolin; J A Steitz
Journal:  Nature       Date:  1980-01-10       Impact factor: 49.962

9.  Comparison of total sequence of a cloned rabbit beta-globin gene and its flanking regions with a homologous mouse sequence.

Authors:  A van Ooyen; J van den Berg; N Mantei; C Weissmann
Journal:  Science       Date:  1979-10-19       Impact factor: 47.728

10.  The enzymatic preparation of [alpha-32P]ATP, [alpha-32P]GTP, [32P]cAMP, and [32P]cGMP, and their use in the assay of adenylate and guanylate cyclases and cyclic nucleotide phosphodiesterases.

Authors:  R A Johnson; T F Walseth
Journal:  Adv Cyclic Nucleotide Res       Date:  1979
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  44 in total

1.  Effect of 5' splice site mutations on splicing of the preceding intron.

Authors:  M Talerico; S M Berget
Journal:  Mol Cell Biol       Date:  1990-12       Impact factor: 4.272

2.  In vivo recognition of a vertebrate mini-exon as an exon-intron-exon unit.

Authors:  D A Sterner; S M Berget
Journal:  Mol Cell Biol       Date:  1993-05       Impact factor: 4.272

3.  The 5' and 3' splice sites come together via a three dimensional diffusion mechanism.

Authors:  Z Pasman; M A Garcia-Blanco
Journal:  Nucleic Acids Res       Date:  1996-05-01       Impact factor: 16.971

4.  Exon definition may facilitate splice site selection in RNAs with multiple exons.

Authors:  B L Robberson; G J Cote; S M Berget
Journal:  Mol Cell Biol       Date:  1990-01       Impact factor: 4.272

5.  The length but not the sequence of the polyoma virus late leader exon is important for both late RNA splicing and stability.

Authors:  G R Adami; G G Carmichael
Journal:  Nucleic Acids Res       Date:  1987-03-25       Impact factor: 16.971

6.  Aberrant splicing events that are induced by proviral integration: implications for myb oncogene activation.

Authors:  D Rosson; D Dugan; E P Reddy
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

7.  The length of the downstream exon and the substitution of specific sequences affect pre-mRNA splicing in vitro.

Authors:  P J Furdon; R Kole
Journal:  Mol Cell Biol       Date:  1988-02       Impact factor: 4.272

8.  In vitro splicing of simian virus 40 early pre mRNA.

Authors:  J C Noble; C Prives; J L Manley
Journal:  Nucleic Acids Res       Date:  1986-02-11       Impact factor: 16.971

9.  Size and position of intervening sequences are critical for the splicing efficiency of pre-mRNA in the yeast Saccharomyces cerevisiae.

Authors:  F J Klinz; D Gallwitz
Journal:  Nucleic Acids Res       Date:  1985-06-11       Impact factor: 16.971

10.  Splice site selection in polyomavirus late pre-mRNA processing.

Authors:  D B Batt; L M Rapp; G G Carmichael
Journal:  J Virol       Date:  1994-03       Impact factor: 5.103
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