Literature DB >> 6314647

Induction of neutralizing antibodies by all three structural poliovirus polypeptides.

R Dernick, J Heukeshoven, M Hilbrig.   

Abstract

Structural polypeptides VP1, VP2, and VP3 of poliovirus, type 1, strain Mahoney, induced low, but clearly demonstrable levels of neutralizing antibodies in rabbits. Half-neutralization titers of antisera for 1000 PFU/ml of poliovirus were in the range of 0.5 to 1.5 log10 units. Polypeptides were isolated by three independent procedures including different dissociation and separation methods to circumvent their denaturation and possible contamination by neighboring fractions. The best and fastest method was dissociation of poliovirus by formic acid and separation of polypeptides by reverse-phase high-performance liquid chromatography.

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Year:  1983        PMID： 6314647     DOI： 10.1016/0042-6822(83)90134-4

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  10 in total

1.  Molecular basis for linkage of a continuous and discontinuous neutralization epitope on the structural polypeptide VP2 of poliovirus type 1.

Authors:  K J Wiegers; K Wetz; R Dernick
Journal:  J Virol       Date:  1990-03       Impact factor: 5.103

2.  Mutations conferring resistance to neutralization with monoclonal antibodies in type 1 poliovirus can be located outside or inside the antibody-binding site.

Authors:  B Blondel; R Crainic; O Fichot; G Dufraisse; A Candrea; D Diamond; M Girard; F Horaud
Journal:  J Virol       Date:  1986-01       Impact factor: 5.103

3.  Different virus-precipitating activities of neutralizing monoclonal antibodies that recognize distinct sites of poliovirus particles.

Authors:  K Taniguchi; S Urasawa
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574

4.  Binding of neutralizing monoclonal antibodies to empty capsids of poliovirus can be blocked by monospecific antisera to structural polypeptides VP1 and VP2.

Authors:  H Uhlig; F Haardt; R Dernick
Journal:  Arch Virol       Date:  1985       Impact factor: 2.574

5.  Regions of poliovirus protein VP1 produced in Escherichia coli induce neutralizing antibodies.

Authors:  M E Hoatlin; O M Kew; M E Renz
Journal:  J Virol       Date:  1987-05       Impact factor: 5.103

6.  Evidence for at least two dominant neutralization antigens on human rhinovirus 14.

Authors:  B Sherry; R Rueckert
Journal:  J Virol       Date:  1985-01       Impact factor: 5.103

7.  Neutralization of poliovirus by polyclonal antibodies requires binding of a single IgG molecule per virion.

Authors:  K Wetz; P Willingmann; H Zeichhardt; K O Habermehl
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

8.  Identification of a new neutralization antigenic site on poliovirus coat protein VP2.

Authors:  E A Emini; B A Jameson; E Wimmer
Journal:  J Virol       Date:  1984-11       Impact factor: 5.103

9.  Isolation by high-performance liquid chromatography and partial characterization of a 57,000-dalton herpes simplex virus type 1 polypeptide.

Authors:  S Welling-Wester; T Popken-Boer; J B Wilterdink; J van Beeumen; G W Welling
Journal:  J Virol       Date:  1985-05       Impact factor: 5.103

10.  Isolation and immunizations with hepatitis A viral structural proteins: induction of antiprotein, antiviral, and neutralizing responses.

Authors:  J V Hughes; L W Stanton
Journal:  J Virol       Date:  1985-08       Impact factor: 5.103
  10 in total

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