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Literature DB >> 6313617

Characterization of a Rhodospirillum rubrum plasmid: loss of photosynthetic growth in plasmidless strains.

Abstract

A single plasmid of 55 kilobases was found in crude cell lysates of each of nine strains of Rhodospirillum rubrum. Restriction endonuclease analysis showed identical fragment patterns with a given nuclease for all plasmids except one, for which an additional EcoRI site was observed. Elimination of the plasmid required that the cells be passaged several times in 25 mM calcium-containing medium, followed by at least two passages under photosynthetic growth conditions in low-calcium medium before treatment with ethyl methanesulfonate. The resulting plasmidless mutants only grew aerobically and were all incapable of pigment formation and photosynthetic growth, suggesting that plasmid DNA is required for photosynthetic competence in R. rubrum.

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Year: 1983 PMID： 6313617 PMCID： PMC217890 DOI： 10.1128/jb.156.2.737-742.1983

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

13 in total

1. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.

Authors: M Thomas; R W Davis
Journal: J Mol Biol Date: 1975-01-25 Impact factor: 5.469

2. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria.

Authors: T Eckhardt
Journal: Plasmid Date: 1978-09 Impact factor: 3.466

3. General method for the isolation of plasmid deoxyribonucleic acid.

Authors: P Guerry; D J LeBlanc; S Falkow
Journal: J Bacteriol Date: 1973-11 Impact factor: 3.490

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Authors: K D Gibson; R A Niederman
Journal: Arch Biochem Biophys Date: 1970-12 Impact factor: 4.013

5. A simple method for the preparation of large quantities of pure plasmid DNA.

Authors: G O Humphreys; G A Willshaw; E S Anderson
Journal: Biochim Biophys Acta Date: 1975-04-02

6. Mutants of Rhodospirrillum rubrum obtained after long-term anaerobic, dark growth.

Authors: R L Uffen; C Sybesma; R S Wolfe
Journal: J Bacteriol Date: 1971-12 Impact factor: 3.490

7. Satellite DNA in photosynthetic bacteria.

Authors: Y Suyama; J Gibson
Journal: Biochem Biophys Res Commun Date: 1966-08-23 Impact factor: 3.575

8. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.

Authors: J A Meyers; D Sanchez; L P Elwell; S Falkow
Journal: J Bacteriol Date: 1976-09 Impact factor: 3.490

9. Evidence for a disseminated plasmid in Streptococcus mutans.

Authors: F L Macrina; C L Scott
Journal: Infect Immun Date: 1978-04 Impact factor: 3.441

10. Mobilization of the genes for photosynthesis from Rhodopseudomonas capsulata by a promiscuous plasmid.

Authors: B Marrs
Journal: J Bacteriol Date: 1981-06 Impact factor: 3.490

7 in total

1. Cell-cycle-specific fluctuation in cytoplasmic membrane composition in aerobically grown Rhodospirillum rubrum.

Authors: C R Myers; M L Collins
Journal: J Bacteriol Date: 1987-12 Impact factor: 3.490

2. Sequence analysis of the cryptic plasmid pMG101 from Rhodopseudomonas palustris and construction of stable cloning vectors.

Authors: M Inui; J H Roh; K Zahn; H Yukawa
Journal: Appl Environ Microbiol Date: 2000-01 Impact factor: 4.792

7. Complementation of a pleiotropic Nif-Gln regulatory mutant of Rhodospirillum rubrum by a previously unrecognized Azotobacter vinelandii regulatory locus.

Authors: C Z Hu; D C Yoch
Journal: Arch Microbiol Date: 1990 Impact factor: 2.552