Literature DB >> 6312929

Characterization of the major structural proteins of purified bovine viral diarrhea virus.

M F Coria, M J Schmerr, A W McClurkin.   

Abstract

Bovine viral diarrhea virus (BVDV) was concentrated and purified by a combination of ultrafiltration, hydroextraction using polyethylene glycol and affinity chromatography. A lectin from Crotalaria juncea that has an affinity for galactose was used in the affinity chromatography. Virions of BVDV with classic envelopes were observed by electron microscopy. Four major proteins with estimated molecular weights of 75,000, 66,000, 54,000, and 26,000 were identified in sodium dodecyl sulfate--polyacrylamide gel electrophoresis slab gels. The proteins of 75,000 and 54,000 were glycoproteins as shown by staining with dansyl hydrazine.

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Year:  1983        PMID: 6312929     DOI: 10.1007/bf01311200

Source DB:  PubMed          Journal:  Arch Virol        ISSN: 0304-8608            Impact factor:   2.574


  12 in total

1.  Evaluation of acetylethyleneimine-killed bovine viral diarrhea-mucosal disease virus (BVD) vaccine for prevention of BVD infection of the fetus.

Authors:  A W McClurkin; M F Coria; R L Smith
Journal:  Proc Annu Meet U S Anim Health Assoc       Date:  1975

2.  A sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gels.

Authors:  A E Eckhardt; C E Hayes; I J Goldstein
Journal:  Anal Biochem       Date:  1976-05-21       Impact factor: 3.365

3.  Studies on the substructure of togaviruses. II. Analysis of equine arteritis, rubella, bovine viral diarrhea, and hog cholera viruses.

Authors:  M Horzinek; J Maess; R Laufs
Journal:  Arch Gesamte Virusforsch       Date:  1971

4.  Characterization of bovine viral diarrhea viruses. V. Morphology of characteristic particles studied by electron microscopy.

Authors:  A E Ritchie; A L Fernelius
Journal:  Arch Gesamte Virusforsch       Date:  1969

5.  Comparison of low- and high-passage bovine turbinate cells for assay of bovine viral diarrhea virus.

Authors:  A W McClurkin; E C Pirtle; M F Coria; R L Smith
Journal:  Arch Gesamte Virusforsch       Date:  1974

6.  Separation of structural proteins of bovine viral diarrhea virus after degradation by different splitting techniques. Brief report.

Authors:  J W Frost; B Liess
Journal:  Arch Gesamte Virusforsch       Date:  1973

7.  Characterization of bovine viral diarrhea viruses. I. Determination of buoyant density.

Authors:  A L Fernelius
Journal:  Arch Gesamte Virusforsch       Date:  1968

8.  Analysis of soluble bovine viral diarrhoea virus antigens and serological relationship to virus structural glycoproteins.

Authors:  W Matthaeus
Journal:  Zentralbl Veterinarmed B       Date:  1980

9.  Detection of three polypeptides in preparations of bovine viral diarrhoea virus.

Authors:  W Matthaeus
Journal:  Arch Virol       Date:  1979       Impact factor: 2.574

10.  Bovine viral diarrhea virus: affinity chromatography on Crotalaria juncea lectin.

Authors:  J Moreno-Lopez; T Kristiansen; P Kårsnas
Journal:  J Virol Methods       Date:  1981-04       Impact factor: 2.014

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  5 in total

1.  Molecular cloning of complementary DNA from a pneumopathic strain of bovine viral diarrhea virus and its diagnostic application.

Authors:  K V Brock; D A Brian; B T Rouse; L N Potgieter
Journal:  Can J Vet Res       Date:  1988-10       Impact factor: 1.310

2.  Variation in the intracellular polypeptide profiles from different isolates of bovine virus diarrhoea virus.

Authors:  D H Pocock; C J Howard; M C Clarke; J Brownlie
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574

3.  Antigenic variations in bovine viral diarrhea viruses detected by monoclonal antibodies.

Authors:  W Xue; F Blecha; H C Minocha
Journal:  J Clin Microbiol       Date:  1990-08       Impact factor: 5.948

4.  Microarray chip based identification of a mixed infection of bovine herpesvirus 1 and bovine viral diarrhea 2 from Indian cattle.

Authors:  Barkha Ratta; Brijesh Singh Yadav; Mayank Pokhriyal; Meeta Saxena; Bhaskar Sharma
Journal:  Curr Microbiol       Date:  2013-09-12       Impact factor: 2.188

Review 5.  Molecular biology of bovine viral diarrhea virus and its interactions with the host.

Authors:  R O Donis
Journal:  Vet Clin North Am Food Anim Pract       Date:  1995-11       Impact factor: 3.357

  5 in total

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