Literature DB >> 6262351

Bovine viral diarrhea virus: affinity chromatography on Crotalaria juncea lectin.

J Moreno-Lopez, T Kristiansen, P Kårsnas.   

Abstract

Attempts were made to purify bovine viral diarrhea virus by chromatography on Crotalaria juncea lectin coupled to Sepharose 2B. A recovery of abut 65% of viral infectivity after desorption was obtained. Electron microscopy revealed mostly de-enveloped particles, rather uniform in appearance but differing in size. Immunodiffusion tests with immune calf sera showed precipitation lines of identity between the desorbed virus and extracts from infected cell cultures.

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Year:  1981        PMID: 6262351     DOI: 10.1016/0166-0934(81)90028-8

Source DB:  PubMed          Journal:  J Virol Methods        ISSN: 0166-0934            Impact factor:   2.014


  2 in total

1.  Molecular cloning of complementary DNA from a pneumopathic strain of bovine viral diarrhea virus and its diagnostic application.

Authors:  K V Brock; D A Brian; B T Rouse; L N Potgieter
Journal:  Can J Vet Res       Date:  1988-10       Impact factor: 1.310

2.  Characterization of the major structural proteins of purified bovine viral diarrhea virus.

Authors:  M F Coria; M J Schmerr; A W McClurkin
Journal:  Arch Virol       Date:  1983       Impact factor: 2.574

  2 in total

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