Literature DB >> 6311160

A recording viscometer for assaying mammalian collagenase.

H W Macartney, S R Bates, N L Blumson, D Nelson, D Jamison, D T Elmore.   

Abstract

A recording viscometer for monitoring the action of mammalian collagenase on soluble collagen is described. For this system, where only one peptide bond is cleaved per subunit, it is shown theoretically that the decrease in viscosity is proportional to the fraction of molecules cleaved. Experimental confirmation was obtained by parallel monitoring of hydrolysis by using the fluorescamine assay of liberated amino groups. The initial velocity of reaction is proportional to substrate concentration and enzyme concentration.

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Year:  1983        PMID： 6311160      PMCID： PMC1152120          DOI： 10.1042/bj2130275

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  Collagenase production by human skin fibroblasts.

Authors:  E A Bauer; G P Stricklin; J J Jeffrey; A Z Eisen
Journal:  Biochem Biophys Res Commun       Date:  1975-05-05       Impact factor: 3.575

2.  EXTRACTION OF COLLAGEN FROM CONNECTIVE TISSUE BY NEUTRAL SALT SOLUTIONS.

Authors:  J Gross; J H Highberger; F O Schmitt
Journal:  Proc Natl Acad Sci U S A       Date:  1955-01-15       Impact factor: 11.205

3.  Purification, characterization and inhibition of human skin collagenase.

Authors:  D E Woolley; R W Glanville; D R Roberts; J M Evanson
Journal:  Biochem J       Date:  1978-02-01       Impact factor: 3.857

4.  Purification and characterization of a collagenase extracted from rabbit tumours.

Authors:  P A McCroskery; J F Richards; E D Harris
Journal:  Biochem J       Date:  1975-10       Impact factor: 3.857

5.  A fluorescent assay for proteolytic enzymes.

Authors:  C Schwabe
Journal:  Anal Biochem       Date:  1973-06       Impact factor: 3.365

6.  Human skin collagenase. Isolation and mechanism of attack on the collagen molecule.

Authors:  A Z Eisen; J J Jeffrey; J Gross
Journal:  Biochim Biophys Acta       Date:  1968-03-25

Review 7.  The determination of collagen and elastin.

Authors:  D S Jackson; E G Cleary
Journal:  Methods Biochem Anal       Date:  1967

8.  An improved viscosimetric assay for vertebrate collagenase activity.

Authors:  C Salerno; A Giacomello; P M Fasella
Journal:  Physiol Chem Phys       Date:  1980

9.  Flow-through viscometer for use in the automated determination of hydrolytic enzyme activities: application in protease, amylase, and pectinase assays.

Authors:  J Kuiper; J A Roels; M H Zuidweg
Journal:  Anal Biochem       Date:  1978-10-01       Impact factor: 3.365

10.  Human collagenase: identification and characterization of an enzyme from rheumatoid synovium in culture.

Authors:  J M Evanson; J J Jeffrey; S M Krane
Journal:  Science       Date:  1967-10-27       Impact factor: 47.728
  10 in total
  1 in total

1.  Competitive inhibition of human skin collagenase by N-benzyloxycarbonyl-L-prolyl-L-alanyl-3-amino-2-oxopropyl-L-leucyl-L- alanylglycine ethyl ester.

Authors:  D A Wallace; S R Bates; B Walker; G Kay; J White; D J Guthrie; N L Blumson; D T Elmore
Journal:  Biochem J       Date:  1986-11-01       Impact factor: 3.857
  1 in total

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