Literature DB >> 6310326

Genetic applications of yeast transformation with linear and gapped plasmids.

T L Orr-Weaver, J W Szostak, R J Rothstein.   

Abstract

Techniques for high frequency yeast transformation have been described. A double-strand break introduced by restriction enzyme cleavage can be used to direct a plasmid to integrate into a particular chromosomal locus. Plasmids containing a double-strand gap can be used in a straightforward method for the isolation and mapping of chromosomal alleles. These techniques extend the genetic applications of yeast transformation.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6310326     DOI： 10.1016/0076-6879(83)01017-4

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  235 in total

1.  Cbk1p, a protein similar to the human myotonic dystrophy kinase, is essential for normal morphogenesis in Saccharomyces cerevisiae.

Authors:  W J Racki; A M Bécam; F Nasr; C J Herbert
Journal:  EMBO J       Date:  2000-09-01       Impact factor: 11.598

2.  Evidence for the involvement of the Glc7-Reg1 phosphatase and the Snf1-Snf4 kinase in the regulation of INO1 transcription in Saccharomyces cerevisiae.

Authors:  M K Shirra; K M Arndt
Journal:  Genetics       Date:  1999-05       Impact factor: 4.562

3.  Interchromosomal and intrachromosomal recombination in rad 18 mutants of Saccharomyces cerevisiae.

Authors:  R H Schiestl; R D Gietz; P J Hastings; U Wintersberger
Journal:  Mol Gen Genet       Date:  1990-06

4.  Essential role for diacylglycerol in protein transport from the yeast Golgi complex.

Authors:  B G Kearns; T P McGee; P Mayinger; A Gedvilaite; S E Phillips; S Kagiwada; V A Bankaitis
Journal:  Nature       Date:  1997-05-01       Impact factor: 49.962

5.  Effect of rad50 mutation on illegitimate recombination in Saccharomyces cerevisiae.

Authors:  Cecilia Y Chan; Jie Zhu; Robert H Schiestl
Journal:  Mol Genet Genomics       Date:  2011-04-22       Impact factor: 3.291

6.  Contribution of domain structure to the RNA 3' end processing and degradation functions of the nuclear exosome subunit Rrp6p.

Authors:  Seasson Phillips; J Scott Butler
Journal:  RNA       Date:  2003-09       Impact factor: 4.942

7.  Design of temperature-sensitive mutants solely from amino acid sequence.

Authors:  Ghadiyaram Chakshusmathi; Kajari Mondal; G Santosh Lakshmi; Guramrit Singh; Ankita Roy; Ravindra Babu Ch; S Madhusudhanan; Raghavan Varadarajan
Journal:  Proc Natl Acad Sci U S A       Date:  2004-05-17       Impact factor: 11.205

8.  Phenotypic consequences of tubulin overproduction in Saccharomyces cerevisiae: differences between alpha-tubulin and beta-tubulin.

Authors:  B Weinstein; F Solomon
Journal:  Mol Cell Biol       Date:  1990-10       Impact factor: 4.272

9.  The fission yeast SEL1 domain protein Cfh3p: a novel regulator of the glucan synthase Bgs1p whose function is more relevant under stress conditions.

Authors:  Mohammad Reza Sharifmoghadam; M-Henar Valdivieso
Journal:  J Biol Chem       Date:  2009-02-23       Impact factor: 5.157

10.  Casein kinase II mediates multiple phosphorylation of Saccharomyces cerevisiae eIF-2 alpha (encoded by SUI2), which is required for optimal eIF-2 function in S. cerevisiae.

Authors:  L Feng; H Yoon; T F Donahue
Journal:  Mol Cell Biol       Date:  1994-08       Impact factor: 4.272
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.