Literature DB >> 6308637

Identification of Escherichia coli DNA helicase I as the traI gene product of the F sex factor.

M Abdel-Monem, G Taucher-Scholz, M Q Klinkert.   

Abstract

Active DNA helicase I (Mr 180,000) can be isolated from Escherichia coli F+ strains but not F- strains. The transfer of the F sex factor to F- strains by conjugation permits the purification of the enzyme from the transconjugant strains. We conclude from this that helicase I is coded for by a portion of the F factor. Results also obtained by using recombinant plasmids carrying different DNA fragments of the F factor transfer region suggest that DNA helicase I is identical to the product of traI, one of the transfer genes of the F factor.

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Year:  1983        PMID： 6308637      PMCID： PMC384103          DOI： 10.1073/pnas.80.15.4659

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  Molecular cloning of DNA from F sex factor of Escherichia coli K-12.

Authors:  R A Skurray; H Nagaishi; A J Clark
Journal:  Proc Natl Acad Sci U S A       Date:  1976-01       Impact factor: 11.205

2.  The requirements for conjugal DNA synthesis in the donor strain during flac transfer.

Authors:  A Kingsman; N Willetts
Journal:  J Mol Biol       Date:  1978-07-05       Impact factor: 5.469

3.  DNA unwinding enzyme II of Escherichia coli. 2. Characterization of the DNA unwinding activity.

Authors:  M Abdel-Monem; H Dürwald; H Hoffmann-Berling
Journal:  Eur J Biochem       Date:  1977-09-15

4.  Enzymic unwinding of DNA. 2. Chain separation by an ATP-dependent DNA unwinding enzyme.

Authors:  M Abdel-Monem; H Dürwald; H Hoffmann-Berling
Journal:  Eur J Biochem       Date:  1976-06-01

5.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

6.  Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.

Authors:  V M Vogt
Journal:  Eur J Biochem       Date:  1973-02-15

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

9.  Enzymic unwinding of DNA. 1. Purification and characterization of a DNA-dependent ATPase from Escherichia coli.

Authors:  M Abdel-Monem; H Hoffmann-Berling
Journal:  Eur J Biochem       Date:  1976-06-01

10.  A mechanism of duplex DNA replication revealed by enzymatic studies of phage phi X174: catalytic strand separation in advance of replication.

Authors:  J F Scott; S Eisenberg; L L Bertsch; A Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1977-01       Impact factor: 11.205
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  31 in total

1.  Single-stranded DNA binding by F TraI relaxase and helicase domains is coordinately regulated.

Authors:  Lubomír Dostál; Joel F Schildbach
Journal:  J Bacteriol       Date:  2010-04-30       Impact factor: 3.490

2.  The F-plasmid TraI protein contains three functional domains required for conjugative DNA strand transfer.

Authors:  Steven W Matson; Heather Ragonese
Journal:  J Bacteriol       Date:  2005-01       Impact factor: 3.490

3.  Structure and function of conjugative pili: monoclonal antibodies as probes for structural variants of F pili.

Authors:  T H Grossman; L S Frost; P M Silverman
Journal:  J Bacteriol       Date:  1990-03       Impact factor: 3.490

4.  traY and traI are required for oriT-dependent enhanced recombination between lac-containing plasmids and lambda plac5.

Authors:  J R Carter; R D Porter
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490

5.  Functional characterization of the multidomain F plasmid TraI relaxase-helicase.

Authors:  Yuan Cheng; Dan E McNamara; Michael J Miley; Rebekah P Nash; Matthew R Redinbo
Journal:  J Biol Chem       Date:  2011-02-02       Impact factor: 5.157

Review 6.  DNA-protein interactions during replication of genetic elements of bacteria.

Authors:  J Nesvera; J Hochmannová
Journal:  Folia Microbiol (Praha)       Date:  1985       Impact factor: 2.099

7.  Revised genetic map of the distal end of the F transfer operon: implications for DNA helicase I, nicking at oriT, and conjugal DNA transport.

Authors:  B A Traxler; E G Minkley
Journal:  J Bacteriol       Date:  1987-07       Impact factor: 3.490

8.  Origin of transfer of IncF plasmids and nucleotide sequences of the type II oriT, traM, and traY alleles from ColB4-K98 and the type IV traY allele from R100-1.

Authors:  B B Finlay; L S Frost; W Paranchych
Journal:  J Bacteriol       Date:  1986-10       Impact factor: 3.490

9.  Characterization of the Escherichia coli F factor traY gene product and its binding sites.

Authors:  W C Nelson; B S Morton; E E Lahue; S W Matson
Journal:  J Bacteriol       Date:  1993-04       Impact factor: 3.490

10.  Double helicase II (uvrD)-helicase IV (helD) deletion mutants are defective in the recombination pathways of Escherichia coli.

Authors:  V M Mendonca; K Kaiser-Rogers; S W Matson
Journal:  J Bacteriol       Date:  1993-08       Impact factor: 3.490
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