Literature DB >> 6307282

Nitrogenase from Klebsiella pneumoniae. An e.p.r. signal observed during enzyme turnover under ethylene is associated with the iron-molybdenum cofactor.

T R Hawkes, D J Lowe, B E Smith.   

Abstract

During turnover at 10 degrees C at pH 7.4 in the presence of ethylene, the MoFe protein of Klebsiella pneumoniae nitrogenase (Kp 1) exhibited an electron-paramagnetic-resonance signal with g-values at 2.12, 1.998 and 1.987. 57Fe isotopic substitution demonstrated that this signal arose from the Kp 1 FeMo-cofactor in an S = 1/2 spin state.

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Year:  1983        PMID： 6307282      PMCID： PMC1154383          DOI： 10.1042/bj2110495

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  13 in total

Review 1.  Structure and function of nitrogenase.

Authors:  L E Mortenson; R N Thorneley
Journal:  Annu Rev Biochem       Date:  1979       Impact factor: 23.643

2.  Novel metal cluster in the iron-molybdenum cofactor of nitrogenase. Spectroscopic evidence.

Authors:  J Rawlings; V K Shah; J R Chisnell; W J Brill; R Zimmermann; E Münck; W H Orme-Johnson
Journal:  J Biol Chem       Date:  1978-02-25       Impact factor: 5.157

3.  Electron-paramagnetic-resonance studies on nitrogenase of Klebsiella pneumoniae. Evidence for acetylene- and ethylene-nitrogenase transient complexes.

Authors:  D J Lowe; R R Eady; N F Thorneley
Journal:  Biochem J       Date:  1978-07-01       Impact factor: 3.857

4.  Identification of iron-sulfur centers in the iron-molybdenum proteins of nitrogenase.

Authors:  D M Kurtz; R S McMillan; B K Burgess; L E Mortenson; R H Holm
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

5.  Studies by electron paramagnetic resonance on the catalytic mechanism of nitrogenase of Klebsiella pneumoniae.

Authors:  B E Smith; D J Lowe; R C Bray
Journal:  Biochem J       Date:  1973-10       Impact factor: 3.857

6.  Nitrogenase of Klebsiella pneumoniae. Purification and properties of the component proteins.

Authors:  R R Eady; B E Smith; K A Cook; J R Postgate
Journal:  Biochem J       Date:  1972-07       Impact factor: 3.857

7.  Spin-spin interaction between molybdenum and one of the iron-sulphur systems of xanthine oxidase and its relevance to the enzymic mechanism.

Authors:  D J Lowe; R M Lynden-Bell; R C Bray
Journal:  Biochem J       Date:  1972-11       Impact factor: 3.857

8.  Isolation of an iron-molybdenum cofactor from nitrogenase.

Authors:  V K Shah; W J Brill
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205

9.  Large-scale purification of high activity Azotobacter vinelandII nitrogenase.

Authors:  B K Burgess; D B Jacobs; E I Stiefel
Journal:  Biochim Biophys Acta       Date:  1980-07-10

10.  Mössbauer spectroscopy of the nitrogenase proteins from Klebsiella pneumoniae. Structural assignments and mechanistic conclusions.

Authors:  B E Smith; G Lang
Journal:  Biochem J       Date:  1974-02       Impact factor: 3.857
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  3 in total

1.  The inactive MoFe protein (NifB-Kp1) of the nitrogenase from nifB mutants of Klebsiella pneumoniae. Its interaction with FeMo-cofactor and the properties of the active MoFe protein formed.

Authors:  T R Hawkes; B E Smith
Journal:  Biochem J       Date:  1984-11-01       Impact factor: 3.857

2.  Electron-paramagnetic-resonance and magnetic-circular-dichroism studies of the binding of cyanide and thiols to the thiols to the iron-molybdenum cofactor from Klebsiella pneumoniae nitrogenase.

Authors:  A J Richards; D J Lowe; R L Richards; A J Thomson; B E Smith
Journal:  Biochem J       Date:  1994-01-15       Impact factor: 3.857

Review 3.  The Spectroscopy of Nitrogenases.

Authors:  Casey Van Stappen; Laure Decamps; George E Cutsail; Ragnar Bjornsson; Justin T Henthorn; James A Birrell; Serena DeBeer
Journal:  Chem Rev       Date:  2020-04-02       Impact factor: 60.622
  3 in total

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