Literature DB >> 6306588

Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe.

I Schwartz, R A Klotsky, D Elseviers, P J Gallagher, M Krauskopf, M A Siddiqui, J F Wong, B A Roe.   

Abstract

A recombinant plasmid (designated pID2) carrying the E. coli gene for tRNAPhe has been isolated from a plasmid bank constructed by the ligation of a total EcoRI digest of E. coli K12 DNA into the EcoRI site of pACYC184 DNA. The plasmid was selected by virtue of its ability to complement a temperature-sensitive lesion in the gene (PheS) for the alpha-subunit of phenylalanyl-tRNA synthetase. Crude tRNA isolated from such transformants exhibited elevated levels of phenylalanine acceptor activity. The tRNAPhe gene has been localized within the first 300 base pairs of a 3.6 kb SalI fragment of pID2. The sequence of the gene and its flanking regions is presented.

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Year:  1983        PMID: 6306588      PMCID: PMC326053          DOI: 10.1093/nar/11.13.4379

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  28 in total

1.  The sequence of phenylalanine tRNA from E. coli.

Authors:  B G. Barrell; F Sanger
Journal:  FEBS Lett       Date:  1969-06       Impact factor: 4.124

2.  Regulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. I. Isolation and characterization of a mutant with elevated levels of tRNAGln 1.

Authors:  S Morgan; A Körner; K B Low; D Söll
Journal:  J Mol Biol       Date:  1977-12-25       Impact factor: 5.469

Review 3.  Regulatory sequences involved in the promotion and termination of RNA transcription.

Authors:  M Rosenberg; D Court
Journal:  Annu Rev Genet       Date:  1979       Impact factor: 16.830

4.  DNA sequences of promoter regions for rRNA operons rrnE and rrnA in E. coli.

Authors:  H A de Boer; S F Gilbert; M Nomura
Journal:  Cell       Date:  1979-05       Impact factor: 41.582

5.  Duplicate genes for tyrosine transfer RNA in Escherichia coli.

Authors:  R L Russell; J N Abelson; A Landy; M L Gefter; S Brenner; J D Smith
Journal:  J Mol Biol       Date:  1970-01-14       Impact factor: 5.469

6.  Nucleotide sequence of an Escherichia coli tRNA (Leu 1) operon and identification of the transcription promoter signal.

Authors:  G Duester; R K Campen; W M Holmes
Journal:  Nucleic Acids Res       Date:  1981-05-11       Impact factor: 16.971

7.  The tyrT locus: termination and processing of a complex transcript.

Authors:  J Rossi; J Egan; L Hudson; A Landy
Journal:  Cell       Date:  1981-11       Impact factor: 41.582

8.  Transcription of the E. coli tufB gene: cotranscription with four tRNA genes and inhibition by guanosine-5'-diphosphate-3'-diphosphate.

Authors:  A Miyajima; M Shibuya; Y Kuchino; Y Kaziro
Journal:  Mol Gen Genet       Date:  1981

9.  Dual function transcripts specifying tRNA and mRNA.

Authors:  L Hudson; J Rossi; A Landy
Journal:  Nature       Date:  1981-12-03       Impact factor: 49.962

10.  PROTEIN AND NUCLEIC ACID SYNTHESIS IN TWO MUTANTS OF ESCHERICHIA COLI WITH TEMPERATURE-SENSITIVE AMINOACYL RIBONUCLEIC ACID SYNTHETASES.

Authors:  L EIDLIC; F C NEIDHARDT
Journal:  J Bacteriol       Date:  1965-03       Impact factor: 3.490

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  23 in total

1.  Identification of the pheS5 mutation, which causes thermosensitivity of Escherichia coli mutant NP37.

Authors:  P Kast; B Keller; H Hennecke
Journal:  J Bacteriol       Date:  1992-03       Impact factor: 3.490

2.  Growth rate regulation of translation initiation factor IF3 biosynthesis in Escherichia coli.

Authors:  D Liveris; R A Klotsky; I Schwartz
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

3.  Palindromic units are part of a new bacterial interspersed mosaic element (BIME).

Authors:  E Gilson; W Saurin; D Perrin; S Bachellier; M Hofnung
Journal:  Nucleic Acids Res       Date:  1991-04-11       Impact factor: 16.971

Review 4.  Structure and organization of the transfer ribonucleic acid genes of Escherichia coli K-12.

Authors:  M J Fournier; H Ozeki
Journal:  Microbiol Rev       Date:  1985-12

5.  tRNA anticodon replacement experiments show that ribosomal frameshifting can be caused by doublet decoding.

Authors:  A G Bruce; J F Atkins; R F Gesteland
Journal:  Proc Natl Acad Sci U S A       Date:  1986-07       Impact factor: 11.205

Review 6.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors:  M K Berlyn
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

Review 7.  Linkage map of Escherichia coli K-12, edition 8.

Authors:  B J Bachmann
Journal:  Microbiol Rev       Date:  1990-06

8.  Genetic mapping of pheU, an Escherichia coli gene for phenylalanine tRNA.

Authors:  P J Gallagher; I Schwartz; D Elseviers
Journal:  J Bacteriol       Date:  1984-05       Impact factor: 3.490

9.  Structure of an Escherichia coli tRNA operon containing linked genes for arginine, histidine, leucine, and proline tRNAs.

Authors:  L M Hsu; H J Klee; J Zagorski; M J Fournier
Journal:  J Bacteriol       Date:  1984-06       Impact factor: 3.490

10.  rRNA gene organization in the Lyme disease spirochete, Borrelia burgdorferi.

Authors:  J J Schwartz; A Gazumyan; I Schwartz
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490

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