Literature DB >> 6306163

Replication of human rotavirus in cell culture.

C J Birch, S M Rodger, J A Marshall, I D Gust.   

Abstract

Nine strains of human rotavirus were adapted to growth in CV-1 and/or MA-104 cells following pretreatment of virus with trypsin, incorporation of trypsin into culture medium, and use of roller cultures. Immunofluorescence was the most reliable method for the detection of virus replication, although characteristic cytopathic effects were produced sporadically by most isolates. Virus could be readily detected in supernatant fluids of cell cultures and in cell sections by electron microscopy.

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Year:  1983        PMID： 6306163     DOI： 10.1002/jmv.1890110307

Source DB:  PubMed          Journal:  J Med Virol        ISSN： 0146-6615            Impact factor:   2.327


  13 in total

1.  Rapid screening test for the diagnosis of rotavirus infection.

Authors:  T Chudzio; S Kasatiya; N Irvine; P Sankar-Mistry
Journal:  J Clin Microbiol       Date:  1989-10       Impact factor: 5.948

2.  Physicochemical stability and inactivation of human and simian rotaviruses.

Authors:  Z D Meng; C Birch; R Heath; I Gust
Journal:  Appl Environ Microbiol       Date:  1987-04       Impact factor: 4.792

3.  Culture adaptation and characterization of group A rotaviruses causing diarrheal illnesses in Bangladesh from 1985 to 1986.

Authors:  R L Ward; J D Clemens; D A Sack; D R Knowlton; M M McNeal; N Huda; F Ahmed; M Rao; G M Schiff
Journal:  J Clin Microbiol       Date:  1991-09       Impact factor: 5.948

4.  Detection of rotavirus in human stools by using monoclonal antibody.

Authors:  G Cukor; D M Perron; R Hudson; N R Blacklow
Journal:  J Clin Microbiol       Date:  1984-06       Impact factor: 5.948

5.  Efficiency of human rotavirus propagation in cell culture.

Authors:  R L Ward; D R Knowlton; M J Pierce
Journal:  J Clin Microbiol       Date:  1984-06       Impact factor: 5.948

6.  Radioimmunofocus assay for detection and quantitation of human rotavirus.

Authors:  S Liu; C Birch; A Coulepis; I Gust
Journal:  J Clin Microbiol       Date:  1984-09       Impact factor: 5.948

Review 7.  Human viral gastroenteritis.

Authors:  G Cukor; N R Blacklow
Journal:  Microbiol Rev       Date:  1984-06

8.  Isolation from faecal specimens of new strains of human rotavirus primarily cytopathic for stationary cell cultures without trypsin.

Authors:  A M Aglianó; A Rossi; A Sanna
Journal:  Arch Virol       Date:  1985       Impact factor: 2.574

9.  HT-29 cells: a new substrate for rotavirus growth.

Authors:  F Superti; A Tinari; L Baldassarri; G Donelli
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

10.  Reverse Genetics Approach for Developing Rotavirus Vaccine Candidates Carrying VP4 and VP7 Genes Cloned from Clinical Isolates of Human Rotavirus.

Authors:  Yuta Kanai; Misa Onishi; Takahiro Kawagishi; Pimfhun Pannacha; Jeffery A Nurdin; Ryotaro Nouda; Moeko Yamasaki; Tina Lusiany; Pattara Khamrin; Shoko Okitsu; Satoshi Hayakawa; Hirotaka Ebina; Hiroshi Ushijima; Takeshi Kobayashi
Journal:  J Virol       Date:  2020-12-22       Impact factor: 5.103
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