Isolation from faecal specimens of new strains of human rotavirus primarily cytopathic for stationary cell cultures without trypsin.

Eight cytopathic human rotavirus strains were isolated in LLC-MK2 cells and in human embryo fibroblasts. The strains were isolated from faeces collected from pediatric and adult patients. Pretreatment of specimens with trypsin and trypsin incorporation in maintenance medium were not performed. Inoculated monolayers were not subjected to centrifugation and were incubated stationary at 36 degrees C. Viruses were identified by electron microscopy and by fluorescent antibody techniques. It is suggested that these rotaviruses are different from any previously recovered from man.