Literature DB >> 6304640

The pR UV+ plasmid, transfected into mammalian cells, enhances their UV survival.

R Elli, L Marcucci, R Bosi, C Oppi, P A Battaglia, F Gigliani.   

Abstract

It has been recently reported that the pR plasmid enhances the UV survival in E.coli c600. In order to test whether this function may be expressed also in mammalian cells, LTA (tk- aprt-) mouse cells were cotransformed with pR plasmid DNA and ptk1 plasmid as selectable marker. Tk+ transformants were analyzed for their UV survival and for the presence of pR DNA sequences by blot-hybridization. The results show a correlation between the enhanced UV survival and presence of pR DNA sequences in cotransformed LTA mouse cells.

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Year:  1983        PMID: 6304640      PMCID: PMC325995          DOI: 10.1093/nar/11.11.3679

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  7 in total

1.  Transformation of mammalian cells with genes from procaryotes and eucaryotes.

Authors:  M Wigler; R Sweet; G K Sim; B Wold; A Pellicer; E Lacy; T Maniatis; S Silverstein; R Axel
Journal:  Cell       Date:  1979-04       Impact factor: 41.582

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

4.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

5.  A simple method for the preparation of large quantities of pure plasmid DNA.

Authors:  G O Humphreys; G A Willshaw; E S Anderson
Journal:  Biochim Biophys Acta       Date:  1975-04-02

6.  Identification of a protein coded by pR plasmid and involved in SOS repair in E. coli.

Authors:  F Gigliani; R Elli; L Marcucci; P A Battaglia
Journal:  Nucleic Acids Res       Date:  1981-02-11       Impact factor: 16.971

7.  Expression of a bacterial gene in mammalian cells.

Authors:  R C Mulligan; P Berg
Journal:  Science       Date:  1980-09-19       Impact factor: 47.728

  7 in total
  3 in total

1.  Cytogenetic characterization of a mouse cell line transformed by a bacterial plasmid.

Authors:  P Petrinelli; R Elli; L Marcucci; M Proietti; M Vinci; A Antonelli
Journal:  Cytotechnology       Date:  1987-10       Impact factor: 2.058

2.  Molecular analysis of the UV protection and mutation genes carried by the I incompatibility group plasmid TP110.

Authors:  J A Glazebrook; K K Grewal; P Strike
Journal:  J Bacteriol       Date:  1986-10       Impact factor: 3.490

3.  Cellular response to DNA damage is enhanced by the pR plasmid in mouse cells and in Escherichia coli.

Authors:  L Marcucci; F Gigliani; P A Battaglia; R Bosi; E Sporeno; R Elli
Journal:  Mol Cell Biol       Date:  1986-02       Impact factor: 4.272

  3 in total

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