Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Cloning and expression of the Staphylococcus aureus protein A gene in Escherichia coli.

Literature DB >> 6304620

Cloning and expression of the Staphylococcus aureus protein A gene in Escherichia coli.

Abstract

A gene bank of Staphylococcus aureus strain Cowan I was established using an E. coli HB101/pBR327 host-vector system. Recombinants expressing staphylococcal protein A (SPA) were detected using an IgG-binding assay. A 3.2 Kb DNA fragment directing the synthesis of SPA in E. coli was identified. SPA produced by E. coli was characterised in minicells and by Western blotting and double diffusion experiments.

Entities: Species

Mesh：

Substances：

Year: 1983 PMID： 6304620 PMCID： PMC325948 DOI： 10.1093/nar/11.10.3065

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

19 in total

1. Immunological screening method to detect specific translation products.

Authors: S Broome; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1978-06 Impact factor: 11.205

2. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors: H C Birnboim; J Doly
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

3. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

Review 4. Use of staphylococcal protein A as an immunological reagent.

Authors: J W Goding
Journal: J Immunol Methods Date: 1978 Impact factor: 2.303

5. Transformation and preservation of competent bacterial cells by freezing.

Authors: D A Morrison
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

6. Transformation of chromosomal and plasmid characters in Staphylococcus aureus.

Authors: M Lindberg; J E Sjöström; T Johansson
Journal: J Bacteriol Date: 1972-02 Impact factor: 3.490

7. Localization of protein A in the bacteria.

Authors: J Sjöquist; J Movitz; I B Johansson; H Hjelm
Journal: Eur J Biochem Date: 1972-10-17

8. A radio-immunoelectrophoretic assay for human growth hormone.

Authors: W M Hunter; F C Greenwood
Journal: Biochem J Date: 1964-04 Impact factor: 3.857

9. A simple method for the preparation of large quantities of pure plasmid DNA.

Authors: G O Humphreys; G A Willshaw; E S Anderson
Journal: Biochim Biophys Acta Date: 1975-04-02

10. The transposon Tn1 as a probe for studying ColE1 structure and function.

Authors: G Dougan; D Sherratt
Journal: Mol Gen Genet Date: 1977-03-07

7 in total

1. Expression of the gene encoding protein A in Staphylococcus aureus and coagulase-negative staphylococci.

Authors: M Uhlén; B Guss; B Nilsson; F Götz; M Lindberg
Journal: J Bacteriol Date: 1984-08 Impact factor: 3.490

2. Gene for an immunoglobulin-binding protein from a group G streptococcus.

Authors: S R Fahnestock; P Alexander; J Nagle; D Filpula
Journal: J Bacteriol Date: 1986-09 Impact factor: 3.490

3. Efficient expression of a Trypanosoma cruzi antigen in Escherichia coli and Staphylococcus aureus and its rapid purification.

Authors: J I Moreno; M Seigelchifer; J Zorzópulos
Journal: World J Microbiol Biotechnol Date: 1991-05 Impact factor: 3.312