Application of affinity partitioning in an aqueous two-phase system to the investigation of triazine dye enzyme interactions.

Affinity partitioning in an aqueous two-phase system by using triazine dye-substituted polyethylene glycol and dextran was applied in the investigation of the affinity of kinases, dehydrogenases and aminotransferases to twelve triazine dyes. From the alteration of the partition coefficient (K) of the enzymes in the two-phase system in the presence or absence of dye-liganded polyethylene glycol, expressed as delta log K, quantitative data for the affinity of the enzymes to various triazine dyes were obtained. Optimal conditions for the purification of enzymes by applying dye-protein interaction were derived.