Literature DB >> 3752985

Liquid-liquid extraction of lactate dehydrogenase from muscle using polymer-bound triazine dyes.

G Johansson, M Joelsson.   

Abstract

An extract from porcine muscle containing soluble enzymes has been partitioned between the two liquid phases of an aqueous, biphasic system consisting of dextran, polyethylene glycol, and water. The influence of polymer-bound triazine dyes (Cibacron blue F3G-A and Procion yellow HE-3G) on the partition of lactate dehydrogenase and total protein was studied. The effects of pH and concentrations of polymers and buffer on this so-called affinity partitioning were also determined. The two-phase systems were used in extraction procedures for purification of lactate dehydrogenase to a specific activity of 456-494 U (7.6-8.4 mukat) per mg protein. The use of these systems for extraction of enzymes in technical scale is discussed.

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Year:  1986        PMID： 3752985     DOI： 10.1007/bf02798435

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  15 in total

1.  3-phosphoglycerate kinase of skeletal muscle.

Authors:  R K Scopes
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

2.  The log S-log z diagram for radio galaxies and its relation to cosmology.

Authors:  F Hoyle; G R Burbidge
Journal:  Nature       Date:  1970-07-25       Impact factor: 49.962

3.  Affinity chromatography on immobilized dyes.

Authors:  C R Lowe; J C Pearson
Journal:  Methods Enzymol       Date:  1984       Impact factor: 1.600

4.  Affinity partitioning of phosphofructokinase from baker's yeast using polymer-bound Cibacron blue F3G-A.

Authors:  G Johansson; G Kopperschläger; P A Albertsson
Journal:  Eur J Biochem       Date:  1983-04-05

Review 5.  Phase partition--a method for purification and analysis of cell organelles and membrane vesicles.

Authors:  P A Albertsson; B Andersson; C Larsson; H E Akerlund
Journal:  Methods Biochem Anal       Date:  1982

6.  Affinity partitioning.

Authors:  G Johansson
Journal:  Methods Enzymol       Date:  1984       Impact factor: 1.600

7.  Affinity partitioning with polymer-bound Cibacron blue F3G-A for rapid, large-scale purification of phosphofructokinase from Baker's yeast.

Authors:  G Kopperschläger; G Johansson
Journal:  Anal Biochem       Date:  1982-07-15       Impact factor: 3.365

8.  Application of affinity partitioning in an aqueous two-phase system to the investigation of triazine dye enzyme interactions.

Authors:  G Kopperschläger; G Lorenz; E Usbeck
Journal:  J Chromatogr       Date:  1983-03-25

9.  Histone-hydrocarbon interaction. Partition of histones in aqueous two-phase systems containing poly(ethylene glycol)-bound hydrocarbons.

Authors:  C G Axelsson; V P Shanbhag
Journal:  Eur J Biochem       Date:  1976-12-11

10.  Parameters determining affinity partitioning of yeast enzymes using polymer-bound triazine dye ligands.

Authors:  G Johansson; M Andersson
Journal:  J Chromatogr       Date:  1984-10-26
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  1 in total

Review 1.  Aqueous two-phase extraction and purification of animal proteins.

Authors:  Michael J Boland
Journal:  Mol Biotechnol       Date:  2002-01       Impact factor: 2.695
  1 in total

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