Literature DB >> 6303731

Efficient bacterial expression of bovine and porcine growth hormones.

P H Seeburg, S Sias, J Adelman, H A de Boer, J Hayflick, P Jhurani, D V Goeddel, H L Heyneker.   

Abstract

cDNAs prepared using poly(A)mRNA from pituitaries and containing the coding sequences for bovine and porcine growth hormones (bGH and pGH) were cloned in bacteria. The primary structures of the peptide hormones derived from the nucleotide sequences of the respective cDNAs show approximately 90% homology. The cloned cDNAs were modified using synthetic DNA to construct expression vectors for efficient bacterial production of the mature animal growth hormones.

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Year:  1983        PMID： 6303731     DOI： 10.1089/dna.1.1983.2.37

Source DB:  PubMed          Journal:  DNA        ISSN： 0198-0238


  16 in total

1.  Effect of nucleotide sequences directly downstream from the AUG on the expression of bovine somatotropin in E. coli.

Authors:  C S Tomich; E R Olson; M K Olsen; P S Kaytes; S K Rockenbach; N T Hatzenbuhler
Journal:  Nucleic Acids Res       Date:  1989-04-25       Impact factor: 16.971

2.  Identification of a cloned cytokinin biosynthetic gene.

Authors:  G F Barry; S G Rogers; R T Fraley; L Brand
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

3.  Translation of a synthetic two-cistron mRNA in Escherichia coli.

Authors:  B E Schoner; R M Belagaje; R G Schoner
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

4.  Preparation and characterization of bovine growth hormones produced in recombinant Escherichia coli.

Authors:  P T Wingfield; P Graber; G Buell; K Rose; M G Simona; B D Burleigh
Journal:  Biochem J       Date:  1987-05-01       Impact factor: 3.857

5.  Three-dimensional structure of a genetically engineered variant of porcine growth hormone.

Authors:  S S Abdel-Meguid; H S Shieh; W W Smith; H E Dayringer; B N Violand; L A Bentle
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205

6.  An examination of the generation-time effect on molecular evolution.

Authors:  T Ohta
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-15       Impact factor: 11.205

7.  Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli.

Authors:  B E Schoner; H M Hsiung; R M Belagaje; N G Mayne; R G Schoner
Journal:  Proc Natl Acad Sci U S A       Date:  1984-09       Impact factor: 11.205

8.  Expression of a mutated bovine growth hormone gene suppresses growth of transgenic mice.

Authors:  W Y Chen; D C Wight; T E Wagner; J J Kopchick
Journal:  Proc Natl Acad Sci U S A       Date:  1990-07       Impact factor: 11.205

9.  Expression of the bovine growth hormone alters the root morphology in transgenic tobacco plants.

Authors:  Kyunghee Oh; Ban Yoon Cheon; Sung Hyun Cho; Hoang Quoc Truong; Sung Han Ok; Ji Ung Jeung; Jang Won Choi; Jeong Sheop Shin
Journal:  Transgenic Res       Date:  2003-06       Impact factor: 2.788

10.  Isolation and characterization of phosphorylated bovine prolactin.

Authors:  B G Kim; C L Brooks
Journal:  Biochem J       Date:  1993-11-15       Impact factor: 3.857
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