Literature DB >> 6302167

Chemical linkage of erythrocytes and viral antigen in the hemolysis-in-gel (HIG) test for viral antibodies.

J Steinmann, H J Marzock.   

Abstract

The sensitivity of the hemolysis-in-gel (HIG) test with rubella antigen is not improved by chemical linkage of the virus to the erythrocytes, and after such modification, IgM specific antibodies are not detectable. In the influenza HIG test with tetraazotized o-dianisidine (TOD), chromic chloride and potassium periodate as coupling reagents, increased sensitivity was observed with allantoic fluid of infected eggs as antigen. If Tween-ether treated hemagglutinin is used in the HIG test, zones of hemolysis are detectable only after treatment of the erythrocytes with TOD, chromic chloride and potassium periodate.

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Year:  1983        PMID： 6302167      PMCID： PMC7131419          DOI： 10.1016/0022-1759(83)90034-0

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  16 in total

1.  Quantitation of antibody to non-hemagglutinating viruses by single radial hemolysis: serological test for human coronaviruses.

Authors:  J C Hierholzer; G A Tannock
Journal:  J Clin Microbiol       Date:  1977-06       Impact factor: 5.948

2.  Modification of virus receptors by metaperiodate; the properties of IO4-treated red-cells.

Authors:  S FAZEKAS DE ST GROTH
Journal:  Aust J Exp Biol Med Sci       Date:  1949-01

3.  Glutaraldehyde, cyanuric chloride and tetrazotized O-dianisidine as coupling reagents in the passive hemagglutination test.

Authors:  S Avrameas; B Taudou; S Chuilon
Journal:  Immunochemistry       Date:  1969-01

4.  A single radial haemolysis technique for the measurement of influenza antibody.

Authors:  S M Russell; D McCahon; A S Beare
Journal:  J Gen Virol       Date:  1975-04       Impact factor: 3.891

5.  Single-radial hemolysis as a cost-effective determinant of Rubella antibody status.

Authors:  J M Forger; R F Gilfillan
Journal:  J Clin Microbiol       Date:  1979-01       Impact factor: 5.948

6.  Determination of mumps and influenza antibodies by haemolysis-in-gel.

Authors:  P Väänänen; T Hovi; E P Helle; K Penttinen
Journal:  Arch Virol       Date:  1976       Impact factor: 2.574

7.  Evaluation of the hemolysis-in-gel test for the screening of rubella immunity and the demonstration of recent infection.

Authors:  L Grillner; O Strannegård
Journal:  J Clin Microbiol       Date:  1976-02       Impact factor: 5.948

8.  Hemolysis-in-gel test in immunity surveys and diagnosis of rubella.

Authors:  P Väänänen; A Vaheri
Journal:  J Med Virol       Date:  1979       Impact factor: 2.327

9.  Hemagglutination inhibition, single radial hemolysis, and ELISA tests for the detection of IgG and IgM to rubella virus.

Authors:  H Champsaur; E Dussaix; P Tournier
Journal:  J Med Virol       Date:  1980       Impact factor: 2.327

10.  A rubella epidemic in an unvaccinated pregnant population. I. Screening methods and serological results.

Authors:  K Skaug; J C Ulstrup; B Stray-Pedersen
Journal:  Scand J Infect Dis       Date:  1981
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