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Literature DB >> 6300874

Inhibition of Micrococcus luteus DNA topoisomerase I by UV photoproducts.

Abstract

The activity of Micrococcus luteus DNA topoisomerase I on UV-irradiated supercoiled DNA was studied under either processive or distributive reaction conditions. Changes in DNA structure caused by UV irradiation reduce the rate of DNA relaxation at very low concentration of photoproducts. Under processive conditions the inhibition of the topoisomerase I by photoproducts can be quantitated by measuring the amount of substrate left in the replicative form I band. The mode of action of DNA topoisomerase I was affected by the presence of photoproducts in the DNA substrate, although the ability of the enzyme to form a covalent complex with UV-irradiated supercoiled DNA was not changed. The inhibition of topoisomerase I by UV photoproducts has been compared to the effects of single-stranded DNA and UV-irradiated duplex linear DNA on the enzyme, and the results suggest that the inhibition by photoproducts is caused by changes in the conformation of the supercoil. Our findings indicate the possibility that DNA topoisomerase I plays a role in repair.

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Year: 1983 PMID： 6300874 PMCID： PMC393694 DOI： 10.1073/pnas.80.7.1787

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

30 in total

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

2. Selective inhibition of semiconservative DNA synthesis by nalidixic acid in permeabilized bacteria.

Authors: T J Simon; W E Masker; P C Hanawalt
Journal: Biochim Biophys Acta Date: 1974-05-17

3. Clustering of thymine dimers in ultraviolet irradiated DNA and the long-range transfer of electronic excitation along the molecule.

Authors: N N Shafranovskaya; E N Trifonov; Y S Lazurkin; M D Frank-Kamenetskii
Journal: Nat New Biol Date: 1973-01-10

4. Replication of bacteriophage M13. 8. Differential effects of rifampicin and nalidixic acid on the synthesis of the two strands of M13 duplex DNA.

Authors: H M Fidanián; D S Ray
Journal: J Mol Biol Date: 1974-02-15 Impact factor: 5.469

5. The effect of thymine dimers on DNA:DNA hybridization.

Authors: M Kahn
Journal: Biopolymers Date: 1974-04 Impact factor: 2.505

6. Effect of a single thymine photodimer on the oligodeoxythymidylate-polydeoxyadenylate interaction.

Authors: F N Hayes; D L Williams; R L Ratlift; A J Varghese; C S Rupert
Journal: J Am Chem Soc Date: 1971-09 Impact factor: 15.419

7. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors: H W Boyer; D Roulland-Dussoix
Journal: J Mol Biol Date: 1969-05-14 Impact factor: 5.469

8. Purification and characterization of an omega protein from Micrococcus luteus.

Authors: V T Kung; J C Wang
Journal: J Biol Chem Date: 1977-08-10 Impact factor: 5.157

9. Nalidixic acid inhibition of post-ultraviolet recovery in Escherichia coli K-12: requirement for recBC function.

Authors: M A Purdy; K L Yeilding
Journal: Antimicrob Agents Chemother Date: 1976-07 Impact factor: 5.191

10. Coumermycin A1: A preferential inhibitor of replicative DNA synthesis in Escherichia coli. I. In vivo characterization.

Authors: M J Ryan
Journal: Biochemistry Date: 1976-08-24 Impact factor: 3.162

11 in total

Inhibition of Micrococcus luteus DNA topoisomerase I by UV photoproducts.

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

2. Selective inhibition of semiconservative DNA synthesis by nalidixic acid in permeabilized bacteria.

3. Clustering of thymine dimers in ultraviolet irradiated DNA and the long-range transfer of electronic excitation along the molecule.

4. Replication of bacteriophage M13. 8. Differential effects of rifampicin and nalidixic acid on the synthesis of the two strands of M13 duplex DNA.

5. The effect of thymine dimers on DNA:DNA hybridization.

6. Effect of a single thymine photodimer on the oligodeoxythymidylate-polydeoxyadenylate interaction.

7. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

8. Purification and characterization of an omega protein from Micrococcus luteus.

9. Nalidixic acid inhibition of post-ultraviolet recovery in Escherichia coli K-12: requirement for recBC function.

10. Coumermycin A1: A preferential inhibitor of replicative DNA synthesis in Escherichia coli. I. In vivo characterization.

1. DNA unwinding and inhibition of mouse leukemia L1210 DNA topoisomerase I by intercalators.

2. Effects of DNA-binding drugs on T4 DNA ligase.

3. AMP-dependent DNA relaxation catalyzed by DNA ligase occurs by a nicking-closing mechanism.

Review 4. Biology of bacterial deoxyribonucleic acid topoisomerases.

5. Structural alterations of pathologically or physiologically modified DNA.

6. The effect of Escherichia coli Uvr protein binding on the topology of supercoiled DNA.

7. Mechanism of inhibition of DNA gyrase by analogues of nalidixic acid: the target of the drugs is DNA.

Review 8. Nucleotide excision repair in Escherichia coli.

9. ATP-dependent partitioning of the DNA template into supercoiled domains by Escherichia coli UvrAB.

10. DNA unwinding and inhibition of T4 DNA ligase by anthracyclines.