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Literature DB >> 6300795

Nuclear matrix-DNA complex resulting from EcoR1 digestion of HeLa nucleoids is enriched for DNA replicating forks.

M S Valenzuela, G C Mueller, S Dasgupta.

Abstract

Upon treatment of HeLa nucleoids with EcoR1 restriction enzyme, a minute fraction of the DNA remains attached to the nuclear matrix. This matrix-DNA complex appears to be composed of a unique set of proteins. Electron microscopic analysis of the DNA fragments present shows an enrichment for HeLa DNA replicating forks.Images

Entities: Chemical

Mesh：

Substances：

Year: 1983 PMID： 6300795 PMCID： PMC325868 DOI： 10.1093/nar/11.7.2155

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

23 in total

1. Visualization of a novel junction in bacteriophage lambda DNA.

Authors: M S Valenzuela; R B Inman
Journal: Proc Natl Acad Sci U S A Date: 1975-08 Impact factor: 11.205

2. Isolation and characterization of an RNA-containing nuclear matrix from Tetrahymena macronuclei.

Authors: G Herlan; W A Eckert; W Kaffenberger; F Wunderlich
Journal: Biochemistry Date: 1979-05-01 Impact factor: 3.162

3. Nuclear proteins. I. Electrophoretic comparison of mouse nucleoli, heterochromatin, euchromatin and contractile proteins.

Authors: D E Comings; D C Harris
Journal: Exp Cell Res Date: 1975-11 Impact factor: 3.905

4. Nuclear ghosts: a nonmembranous structural component of mammalian cell nuclei.

Authors: J M Keller; D E Riley
Journal: Science Date: 1976-07-30 Impact factor: 47.728

5. Isolation and characterization of the nuclear matrix in Friend erythroleukemia cells: chromatin and hnRNA interactions with the nuclear matrix.

Authors: B H Long; C Y Huang; A O Pogo
Journal: Cell Date: 1979-12 Impact factor: 41.582

6. Analysis of the attachment of replicating DNA to a nuclear matrix in mammalian interphase nuclei.

Authors: P A Dijkwel; L H Mullenders; F Wanka
Journal: Nucleic Acids Res Date: 1979-01 Impact factor: 16.971

7. Bovine renal glomerular basement membrane. Isolation and characterization of a glycoprotein component.

Authors: M Oho; P Riquetti; B G Hudson
Journal: J Biol Chem Date: 1975-10-10 Impact factor: 5.157

8. Restriction enzyme cleavage of DNA resulting from gently lysed Escherichia coli.

Authors: M S Valenzuela; R B Inman
Journal: Mol Gen Genet Date: 1978-11-09

9. Characterization of nuclear structures containing superhelical DNA.

Authors: P R Cook; I A Brazell; E Jost
Journal: J Cell Sci Date: 1976-11 Impact factor: 5.285

10. Nuclear matrix of HeLa S3 cells. Polypeptide composition during adenovirus infection and in phases of the cell cycle.

Authors: L D Hodge; P Mancini; F M Davis; P Heywood
Journal: J Cell Biol Date: 1977-01 Impact factor: 10.539

6 in total

Nuclear matrix-DNA complex resulting from EcoR1 digestion of HeLa nucleoids is enriched for DNA replicating forks.

1. Visualization of a novel junction in bacteriophage lambda DNA.

2. Isolation and characterization of an RNA-containing nuclear matrix from Tetrahymena macronuclei.

3. Nuclear proteins. I. Electrophoretic comparison of mouse nucleoli, heterochromatin, euchromatin and contractile proteins.

4. Nuclear ghosts: a nonmembranous structural component of mammalian cell nuclei.

5. Isolation and characterization of the nuclear matrix in Friend erythroleukemia cells: chromatin and hnRNA interactions with the nuclear matrix.

6. Analysis of the attachment of replicating DNA to a nuclear matrix in mammalian interphase nuclei.

7. Bovine renal glomerular basement membrane. Isolation and characterization of a glycoprotein component.

8. Restriction enzyme cleavage of DNA resulting from gently lysed Escherichia coli.

9. Characterization of nuclear structures containing superhelical DNA.

10. Nuclear matrix of HeLa S3 cells. Polypeptide composition during adenovirus infection and in phases of the cell cycle.

Review 1. The nuclear matrix--its role in the spatial organization and replication of eukaryotic DNA.

2. Mapping replicational sites in the eucaryotic cell nucleus.

3. Yeast ARS function and nuclear matrix association coincide in a short sequence from the human HPRT locus.

4. Replication forks are underrepresented in chromosomal DNA of Xenopus laevis embryos.

5. Initiation of DNA Replication in the Human Genome.

6. Trapping DNA replication origins from the human genome.