Literature DB >> 6299896

A bacteriophage lambda vector for cloning with BamHI and Sau3A.

S Mizusawa, D F Ward.   

Abstract

A phage lambda cloning vector has been constructed which contains a single site for the restriction endonuclease BamHI. Since Sau3A and Bg/II produce the same cohesive ends as BamHI, this vector can also be used to clone DNA fragments generated with either of these enzymes. We have used this vector to construct an Escherichia coli library using partial digestion with Sau3A. This vector will be most useful for applications requiring genetic analysis of cloned E. coli genes.

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Year:  1982        PMID： 6299896     DOI： 10.1016/0378-1119(82)90200-1

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  42 in total

1.  Probing the ATP-binding site of P1 ParA: partition and repression have different requirements for ATP binding and hydrolysis.

Authors:  E Fung; J Y Bouet; B E Funnell
Journal:  EMBO J       Date:  2001-09-03       Impact factor: 11.598

2.  Transcription-induced cytosine-to-thymine mutations are not dependent on sequence context of the target cytosine.

Authors:  A Beletskii; A S Bhagwat
Journal:  J Bacteriol       Date:  2001-11       Impact factor: 3.490

3.  Isolation, characterization, and sequence of an Escherichia coli heat shock gene, htpX.

Authors:  D Kornitzer; D Teff; S Altuvia; A B Oppenheim
Journal:  J Bacteriol       Date:  1991-05       Impact factor: 3.490

4.  Fnr mutants that activate gene expression in the presence of oxygen.

Authors:  P J Kiley; W S Reznikoff
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

5.  A minor arginine tRNA mutant limits translation preferentially of a protein dependent on the cognate codon.

Authors:  K S Chen; T C Peters; J R Walker
Journal:  J Bacteriol       Date:  1990-05       Impact factor: 3.490

6.  Autogenous control is not sufficient to ensure steady-state growth rate-dependent regulation of the S10 ribosomal protein operon of Escherichia coli.

Authors:  L Lindahl; J M Zengel
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

7.  Increased expression of the bifunctional protein PrlF suppresses overproduction lethality associated with exported beta-galactosidase hybrid proteins in Escherichia coli.

Authors:  D R Kiino; G J Phillips; T J Silhavy
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

8.  Isolation and characterization of rcsB mutations that affect colanic acid capsule synthesis in Escherichia coli K-12.

Authors:  G Gupte; C Woodward; V Stout
Journal:  J Bacteriol       Date:  1997-07       Impact factor: 3.490

9.  Plasmid P1 replication: negative control by repeated DNA sequences.

Authors:  D Chattoraj; K Cordes; A Abeles
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

10.  Cell-division control in Escherichia coli: specific induction of the SOS function SfiA protein is sufficient to block septation.

Authors:  O Huisman; R D'Ari; S Gottesman
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205
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