Literature DB >> 6297461

Underestimation of metabolic rates owing to reincorporation of 14CO2 in the perfused rat liver.

J F Tomera, P G Goetz, W M Rand, H Brunengraber.   

Abstract

(14)CO(2) production by perfused rat livers was simulated by infusing NaH(14)CO(3) into the perfusate. Recovery of label as (14)CO(2) gas + perfusate bicarbonate was 45-85%. Rates of (14)CO(2) exchange in the liver are 3-70 times greater than net rates of CO(2) production. Therefore (14)CO(2) reincorporation can lead to significant underestimations of rates of oxidation of (14)C-labelled substrates in liver.

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Year:  1982        PMID： 6297461      PMCID： PMC1153950          DOI： 10.1042/bj2080231

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  12 in total

1.  Mechanisms of fixation of carbon dioxide by heterotrophes and autotrophs.

Authors:  M F UTTER; H G WOOD
Journal:  Adv Enzymol Relat Subj Biochem       Date:  1951

2.  Carbon-14 tracer studies in the metabolism of isolated rat-liver parenchymal cells under conditions of gluconeogenesis from lactate and pyruvate.

Authors:  G Müllhofer; C Müller; C Von Stetten; E Gruber
Journal:  Eur J Biochem       Date:  1977-05-16

3.  Control of gluconeogenesis from amino acids in the perfused rat liver.

Authors:  L E Mallet; J H Exton; C R Park
Journal:  J Biol Chem       Date:  1969-10-25       Impact factor: 5.157

4.  Fatty acid and 3- -hydroxysterol synthesis in the perfused rat liver. Including measurements on the production of lactate, pyruvate, -hydroxy-butyrate, and acetoacetate by the fed liver.

Authors:  H Brunengraber; M Boutry; J M Lowenstein
Journal:  J Biol Chem       Date:  1973-04-25       Impact factor: 5.157

5.  Control mechanisms of gluconeogenesis and ketogenesis. II. Interactions between fatty acid oxidation and the citric acid cycle in perfused rat liver.

Authors:  J R Williamson; R Scholz; E T Browning
Journal:  J Biol Chem       Date:  1969-09-10       Impact factor: 5.157

6.  Phosphoenolpyruvate carboxykinase (guanosine triphosphate) from rat liver cytosol. Separation of homogeneous forms of the enzyme with high and low activity by chromatography on agarose-hexane-guanosine triphosphate.

Authors:  G Colombo; G M Carlson; H A Lardy
Journal:  Biochemistry       Date:  1978-12-12       Impact factor: 3.162

7.  The enzymatic carboxylation of phosphoenolpyruvate. 3. Investigation of the kinetics and mechanism of the mitochondrial phosphoenolpyruvate carboxykinase-catalyzed reaction.

Authors:  H C Chang; H Maruyama; R S Miller; M D Lane
Journal:  J Biol Chem       Date:  1966-05-25       Impact factor: 5.157

8.  Fatty acid, 3-beta-hydroxysterol, and ketone synthesis in the perfused rat liver. Effects of (--)-hydroxycitrate and oleate.

Authors:  H Brunengraber; M Boutry; J M Lowenstein
Journal:  Eur J Biochem       Date:  1978-01-16

9.  Lipogenesis from ketone bodies in the isolated perfused rat liver. Evidence for the cytosolic activation of acetoacetate.

Authors:  G Endemann; P G Goetz; J Edmond; H Brunengraber
Journal:  J Biol Chem       Date:  1982-04-10       Impact factor: 5.157

10.  Synthesis of liver glycogen from 3-C14-DL-malate: evidence for complete isotopic equilibration of C4-dicarboxylic acids.

Authors:  H D HOBERMAN; A F D'ADAMO
Journal:  J Biol Chem       Date:  1960-04       Impact factor: 5.157
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  6 in total

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Authors:  Christian C Voigt; Karin Sörgel; Jurģis Šuba; Oskars Keišs; Gunārs Pētersons
Journal:  Proc Biol Sci       Date:  2012-06-20       Impact factor: 5.349

2.  Metabolic effects of bacitracin in isolated rat hepatocytes.

Authors:  L Agius; C Wilding; K G Alberti
Journal:  Biochem J       Date:  1983-11-15       Impact factor: 3.857

3.  Quantitative analysis of intermediary metabolism in rat hepatocytes incubated in the presence and absence of ethanol with a substrate mixture including ketoleucine.

Authors:  J M Baranyai; J J Blum
Journal:  Biochem J       Date:  1989-02-15       Impact factor: 3.857

4.  The effect of treatment of the rat with bacterial endotoxin on gluconeogenesis and pyruvate metabolism in subsequently isolated hepatocytes.

Authors:  C G Jones; M A Titheradge
Journal:  Biochem J       Date:  1993-01-01       Impact factor: 3.857

5.  Assessment of the flux of mitochondrial acetyl-CoA in liver and kidney by using the differential production of 14CO2 from tracers of (1-14C)- and (2-14C)-labelled 4-methyl-2-oxovalerate.

Authors:  J F Tomera; R R Kopito; H Brunengraber
Journal:  Biochem J       Date:  1983-01-15       Impact factor: 3.857

6.  Quantitative analysis of intermediary metabolism in hepatocytes incubated in the presence and absence of glucagon with a substrate mixture containing glucose, ribose, fructose, alanine and acetate.

Authors:  M Rabkin; J J Blum
Journal:  Biochem J       Date:  1985-02-01       Impact factor: 3.857
  6 in total

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