Literature DB >> 10637332

Selection of functional tRNA primers and primer binding site sequences from a retroviral combinatorial library: identification of new functional tRNA primers in murine leukemia virus replication.

A H Lund1, M Duch, F S Pedersen.   

Abstract

Retroviral reverse transcription is initiated from a cellular tRNA molecule and all known exogenous isolates of murine leukemia virus utilise a tRNA(Pro)molecule. While several studies suggest flexibility in murine leukemia virus primer utilisation, studies on human immunodeficiency virus and avian retro-viruses have revealed evidence of molecular adapt-ation towards the specific tRNA isoacceptor used as replication primer. In this study, murine leukemia virus tRNA utilisation is investigated by in vivo screening of a retroviral vector combinatorial library with randomised primer binding sites. While most of the selected primer binding sites are complementary to the 3'-end of tRNA((Pro)), we also retrieved PBS sequences matching four other tRNA molecules and demonstrate that Akv murine leukemia virus vectors may efficiently replicate using tRNA(Arg(CCU)), tRNA(Phe(GAA))and a hitherto unknown human tRNA(Ser(CGA)).

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Year:  2000        PMID： 10637332      PMCID： PMC102544          DOI： 10.1093/nar/28.3.791

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  67 in total

1.  Extended minus-strand DNA as template for R-U5-mediated second-strand transfer in recombinational rescue of primer binding site-modified retroviral vectors.

Authors:  J G Mikkelsen; A H Lund; K Dybkaer; M Duch; F S Pedersen
Journal:  J Virol       Date:  1998-03       Impact factor: 5.103

2.  Modified nucleotides of tRNAPro restrict interactions in the binary primer/template complex of M-MuLV.

Authors:  P Fossé; M Mougel; G Keith; E Westhof; B Ehresmann; C Ehresmann
Journal:  J Mol Biol       Date:  1998-02-06       Impact factor: 5.469

Review 3.  Primer tRNAs for reverse transcription.

Authors:  J Mak; L Kleiman
Journal:  J Virol       Date:  1997-11       Impact factor: 5.103

4.  A simple and efficient procedure for generating stable expression libraries by cDNA cloning in a retroviral vector.

Authors:  J R Rayner; T J Gonda
Journal:  Mol Cell Biol       Date:  1994-02       Impact factor: 4.272

5.  Effect of mutations in the nucleocapsid protein (NCp7) upon Pr160(gag-pol) and tRNA(Lys) incorporation into human immunodeficiency virus type 1.

Authors:  Y Huang; A Khorchid; J Wang; M A Parniak; J L Darlix; M A Wainberg; L Kleiman
Journal:  J Virol       Date:  1997-06       Impact factor: 5.103

6.  Placement of tRNA primer on the primer-binding site requires pol gene expression in avian but not murine retroviruses.

Authors:  W Fu; B A Ortiz-Conde; R J Gorelick; S H Hughes; A Rein
Journal:  J Virol       Date:  1997-09       Impact factor: 5.103

7.  Initiation of (-) strand DNA synthesis from tRNA(3Lys) on lentiviral RNAs: implications of specific HIV-1 RNA-tRNA(3Lys) interactions inhibiting primer utilization by retroviral reverse transcriptases.

Authors:  E J Arts; S R Stetor; X Li; J W Rausch; K J Howard; B Ehresmann; T W North; B M Wöhrl; R S Goody; M A Wainberg; S F Grice
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-17       Impact factor: 11.205

8.  Cloning mammalian genes by expression selection of genetic suppressor elements: association of kinesin with drug resistance and cell immortalization.

Authors:  A V Gudkov; A R Kazarov; R Thimmapaya; S A Axenovich; I A Mazo; I B Roninson
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-26       Impact factor: 11.205

9.  Complementarity between 3' terminal nucleotides of tRNA and primer binding site is a major determinant for selection of the tRNA primer used for initiation of HIV-1 reverse transcription.

Authors:  Q Yu; C D Morrow
Journal:  Virology       Date:  1999-02-01       Impact factor: 3.616

10.  Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles.

Authors:  J Mak; M Jiang; M A Wainberg; M L Hammarskjöld; D Rekosh; L Kleiman
Journal:  J Virol       Date:  1994-04       Impact factor: 5.103
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  2 in total

1.  tRNASer(CGA) differentially regulates expression of wild-type and codon-modified papillomavirus L1 genes.

Authors:  Wenyi Gu; Mengrong Li; Wei Ming Zhao; Ning Xia Fang; Shurui Bu; Ian H Frazer; Kong-Nan Zhao
Journal:  Nucleic Acids Res       Date:  2004-08-19       Impact factor: 16.971

2.  Forced selection of a human immunodeficiency virus type 1 variant that uses a non-self tRNA primer for reverse transcription: involvement of viral RNA sequences and the reverse transcriptase enzyme.

Authors:  Truus E M Abbink; Nancy Beerens; Ben Berkhout
Journal:  J Virol       Date:  2004-10       Impact factor: 5.103
  2 in total

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