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Literature DB >> 6294610

Gene amplification in methotrexate-resistant mouse cells. IV. Different DNA sequences are amplified in different resistant lines.

Abstract

DNA was purified from double minutes isolated from MTX-resistant EL4/8 mouse lymphoma cells, digested to completion with Bam H1 restriction endonuclease and cloned in lambda-1059. The properties of the library suggest that the DNA from which it was made was not detectably contaminated with non-dm chromosome material, and that the library is essentially complete for sequences contained in Bam H1 restriction fragments between 9 and 19 kb. The inserts of some selected lambda-recombinants were subcloned in pBR328 or pAT153 to separate sequences of differing repetition frequency. Clones representative of different classes of sequences were used as probes to Southern transfers of Bam H1 digested total nuclear DNAs of various MTX-resistant cell lines. The results clearly show that the amplified unit of each cell line has a unique structure, and that different amplified units differ widely in their sequence composition.

Entities: Chemical Disease Gene Species

Mesh：

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Year: 1982 PMID： 6294610 PMCID： PMC326952 DOI： 10.1093/nar/10.21.6597

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

27 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Metaphase chromosome anomaly: association with drug resistance and cell-specific products.

Authors: J L Biedler; B A Spengler
Journal: Science Date: 1976-01-16 Impact factor: 47.728

3. Chromosomes and cancer.

Authors: A Levan; G Levan; F Mitelman
Journal: Hereditas Date: 1977 Impact factor: 3.271

4. Polyoma virus and cyclic AMP-mediated control of dihydrofolate reductase mRNA abundance in methotrexate-resistant mouse fibroblasts.

Authors: R E Kellems; V B Morhenn; E A Pfendt; F W Alt; R T Schimke
Journal: J Biol Chem Date: 1979-01-25 Impact factor: 5.157

5. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors: W D Benton; R W Davis
Journal: Science Date: 1977-04-08 Impact factor: 47.728

6. Loss of resistance to methotrexate in L5178Y mouse leukemia grown in vitro.

Authors: V D Courtenay; A B Robins
Journal: J Natl Cancer Inst Date: 1972-07 Impact factor: 13.506

7. Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.

Authors: F W Alt; R E Kellems; J R Bertino; R T Schimke
Journal: J Biol Chem Date: 1978-03-10 Impact factor: 5.157

8. Amplified dihydrofolate reductase genes are localized to a homogeneously staining region of a single chromosome in a methotrexate-resistant Chinese hamster ovary cell line.

Authors: J H Nunberg; R J Kaufman; R T Schimke; G Urlaub; L A Chasin
Journal: Proc Natl Acad Sci U S A Date: 1978-11 Impact factor: 11.205

9. Nucleotide sequence of the rightward operator of phage lambda.

Authors: T Maniatis; A Jeffrey; D G Kleid
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

10. Correlation of dihydrofolate reductase elevation with gene amplification in a homogeneously staining chromosomal region in L5178Y cells.

Authors: B J Dolnick; R J Berenson; J R Bertino; R J Kaufman; J H Nunberg; R T Schimke
Journal: J Cell Biol Date: 1979-11 Impact factor: 10.539

13 in total

1. Preferential amplification of rearranged sequences near amplified adenylate deaminase genes.

Authors: M Debatisse; I Saito; G Buttin; G R Stark
Journal: Mol Cell Biol Date: 1988-01 Impact factor: 4.272

2. The dihydrofolate reductase amplicons in different methotrexate-resistant Chinese hamster cell lines share at least a 273-kilobase core sequence, but the amplicons in some cell lines are much larger and are remarkably uniform in structure.

Authors: J E Looney; C Ma; T H Leu; W F Flintoff; W B Troutman; J L Hamlin
Journal: Mol Cell Biol Date: 1988-12 Impact factor: 4.272

3. Structure of amplified DNA in different Syrian hamster cell lines resistant to N-(phosphonacetyl)-L-aspartate.

Authors: F Ardeshir; E Giulotto; J Zieg; O Brison; W S Liao; G R Stark
Journal: Mol Cell Biol Date: 1983-11 Impact factor: 4.272

4. Increased dihydrofolate reductase, double minutes and increased nucleolar activation in methotrexate-resistant HeLa cells.

Authors: J G Delinassios; M J Talieri
Journal: Experientia Date: 1983-12-15

5. Isolation of a human genomic fragment, co-amplified with c-Ki-ras, that affects plasmid supercoiling in E. coli.

Authors: J Heighway; A H Geurts van Kessel
Journal: Nucleic Acids Res Date: 1987-04-24 Impact factor: 16.971

6. Cytogenetical changes during early stages of development of methotrexate resistance in HeLa cells.

Authors: J G Delinassios; M J Talieri
Journal: Med Oncol Tumor Pharmacother Date: 1985

7. Co-amplification of double minute chromosomes, multiple drug resistance, and cell surface P-glycoprotein in DNA-mediated transformants of mouse cells.

Authors: S M Robertson; V Ling; C P Stanners
Journal: Mol Cell Biol Date: 1984-03 Impact factor: 4.272

8. Gene amplification in methotrexate-resistant mouse cells. V. Intact amplified units can be transferred to and amplified in methotrexate-sensitive mouse L cells.

Authors: C J Bostock; E M Clark
Journal: Chromosoma Date: 1983 Impact factor: 4.316

9. Characterization of N-myc amplification units in human neuroblastoma cells.

Authors: B A Zehnbauer; D Small; G M Brodeur; R Seeger; B Vogelstein
Journal: Mol Cell Biol Date: 1988-02 Impact factor: 4.272

10. Amplification and expression of human alpha-globin genes in Chinese hamster ovary cells.

Authors: Y F Lau; C C Lin; Y W Kan
Journal: Mol Cell Biol Date: 1984-08 Impact factor: 4.272