Literature DB >> 6293787

Analysis of ligand-receptor interactions with the fluorescence activated cell sorter.

L A Sklar, D A Finney.   

Abstract

The binding of a fluorescent peptide to human neutrophils is analyzed with a fluorescence activated cell sorter. We examine steady-state and kinetic features of the ligand-receptor interaction (in the presence of unbound ligand) and we show tht the number of receptors may be estimated without resorting to any external references for calibration. These methods are applicable to other fluorescent ligands with affinities greater than 10(8) M-1.

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Year:  1982        PMID： 6293787     DOI： 10.1002/cyto.990030304

Source DB:  PubMed          Journal:  Cytometry        ISSN： 0196-4763


  12 in total

Review 1.  Flow cytometry. Principles and applications in exercise immunology.

Authors:  H Gabriel; W Kindermann
Journal:  Sports Med       Date:  1995-11       Impact factor: 11.136

Review 2.  The use of the clinical immunology laboratory.

Authors:  S H Yoshida; B C Veit; L E Mansfield; M E Gershwin
Journal:  Clin Rev Allergy       Date:  1994

3.  Flow cytometry enables a high-throughput homogeneous fluorescent antibody-binding assay for cytotoxic T cell lytic granule exocytosis.

Authors:  Amy E Florian; Christopher K Lepensky; Ohyun Kwon; Mark K Haynes; Larry A Sklar; Adam Zweifach
Journal:  J Biomol Screen       Date:  2012-11-15

4.  Empirically optimized flow cytometric immunoassay validates ambient analyte theory.

Authors:  Zaheer A Parpia; David M Kelso
Journal:  Anal Biochem       Date:  2010-02-10       Impact factor: 3.365

Review 5.  Flow Cytometry: Impact on Early Drug Discovery.

Authors:  Bruce S Edwards; Larry A Sklar
Journal:  J Biomol Screen       Date:  2015-03-24

Review 6.  International Union of Basic and Clinical Pharmacology. LXXIII. Nomenclature for the formyl peptide receptor (FPR) family.

Authors:  Richard D Ye; François Boulay; Ji Ming Wang; Claes Dahlgren; Craig Gerard; Marc Parmentier; Charles N Serhan; Philip M Murphy
Journal:  Pharmacol Rev       Date:  2009-06-04       Impact factor: 25.468

7.  Isoprenoid metabolism is required for stimulation of the respiratory burst oxidase of HL-60 cells.

Authors:  G M Bokoch; V Prossnitz
Journal:  J Clin Invest       Date:  1992-02       Impact factor: 14.808

8.  Mechanism of binding and internalization of ICAM-1-derived cyclic peptides by LFA-1 on the surface of T cells: a potential method for targeted drug delivery.

Authors:  Meagan E Anderson; Teruna J Siahaan
Journal:  Pharm Res       Date:  2003-10       Impact factor: 4.200

9.  A new fluorescent probe for the equilibrative inhibitor-sensitive nucleoside transporter. 5'-S-(2-aminoethyl)-N6-(4-nitrobenzyl)-5'-thioadenosine (SAENTA)-chi 2-fluorescein.

Authors:  J S Wiley; A M Brocklebank; M B Snook; G P Jamieson; W H Sawyer; J D Craik; C E Cass; M J Robins; D P McAdam; A R Paterson
Journal:  Biochem J       Date:  1991-02-01       Impact factor: 3.857

10.  Binding of fluoresceinated epidermal growth factor to A431 cell sub-populations studied using a model-independent analysis of flow cytometric fluorescence data.

Authors:  R C Chatelier; R G Ashcroft; C J Lloyd; E C Nice; R H Whitehead; W H Sawyer; A W Burgess
Journal:  EMBO J       Date:  1986-06       Impact factor: 11.598
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