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Literature DB >> 6292863

A Drosophila ribosomal protein gene is located near repeated sequences including rDNA sequences.

Abstract

We have isolated a cloned segment of Drosophila genomic DNA containing a ribosomal protein gene. Hybridization analysis of the DNA in this clone indicates a complex organization of repeated elements within this cloned segment. At least one of these repeated elements is homologous to regions of rDNA. Restriction analysis of the clone shows that some of the repeated elements are present as tandem duplications and in scattered locations within the cloned DNA segment. There are also three non-ribosomal protein genes contained in this clone, each of which is expressed along with the ribosomal protein gene into RNA species present in Drosophila embryos.

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Year: 1982 PMID： 6292863 PMCID： PMC320944 DOI： 10.1093/nar/10.19.5979

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

18 in total

1. The clustered and scrambled arrangement of moderately repetitive elements in Drosophila DNA.

Authors: P C Wensink; S Tabata; C Pachl
Journal: Cell Date: 1979-12 Impact factor: 41.582

2. Restriction analysis of spacers in ribosomal DNA of Drosophila melanogaster.

Authors: E O Long; I B Dawid
Journal: Nucleic Acids Res Date: 1979-09-11 Impact factor: 16.971

3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

4. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors: G M Wahl; M Stern; G R Stark
Journal: Proc Natl Acad Sci U S A Date: 1979-08 Impact factor: 11.205

5. The use of R-looping for structural gene identification and mRNA purification.

Authors: J L Woolford; M Rosbash
Journal: Nucleic Acids Res Date: 1979-06-11 Impact factor: 16.971

6. Preparative and analytical purification of DNA from agarose.

Authors: B Vogelstein; D Gillespie
Journal: Proc Natl Acad Sci U S A Date: 1979-02 Impact factor: 11.205

7. Construction and identification of cDNA clones for mouse ribosomal proteins: application for the study of r-protein gene expression.

Authors: O Meyuhas; R P Perry
Journal: Gene Date: 1980-07 Impact factor: 3.688

A Drosophila ribosomal protein gene is located near repeated sequences including rDNA sequences.

1. The clustered and scrambled arrangement of moderately repetitive elements in Drosophila DNA.

2. Restriction analysis of spacers in ribosomal DNA of Drosophila melanogaster.

3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

4. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

5. The use of R-looping for structural gene identification and mRNA purification.

6. Preparative and analytical purification of DNA from agarose.

7. Construction and identification of cDNA clones for mouse ribosomal proteins: application for the study of r-protein gene expression.

8. Characterization of six cloned DNAs from Drosophila melanogaster, including one that contains the genes for rRNA.

9. Isolation of cloned DNA sequences containing ribosomal protein genes from Saccharomyces cerevisiae.

10. Isolation and mapping of a cloned ribosomal protein gene of Drosophila melanogaster.

1. A new copia-like transposable element found in a Drosophila rDNA gene unit.