Literature DB >> 6290995

Molecular cloning and characterisation of the two DNA components of tomato golden mosaic virus.

D M Bisaro, W D Hamilton, R H Coutts, K W Buck.   

Abstract

We report the molecular cloning of the tomato golden mosaic virus (TGMV) genome in the E. coli plasmid pAT 153. The results of this work conclusively show that TGMV DNA consists of two components (designated A and B) of almost, but not exactly, the same size. Four different recombinant plasmids are described, two containing component A in opposite orientation and two containing component B in opposite orientation. Southern blot analysis revealed little sequence homology between A and B and showed both components to be equally represented in viral and intracellular DNA forms. Detailed restriction maps of the cloned DNAs are presented, and a comparison of these with digests of intracellular viral dsDNA indicates that the former are full-length faithful copies of TGMV DNA. This is the first report of the cloning of a geminivirus genome.

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Year:  1982        PMID： 6290995      PMCID： PMC320841          DOI： 10.1093/nar/10.16.4913

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Isolation of intact individual species of single- and double-stranded RNA after fractionation by polyacrylamide gel electrophoresis.

Authors:  A R Schuerch; W R Mitchell; W K Joklik
Journal:  Anal Biochem       Date:  1975-05-12       Impact factor: 3.365

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  End labeling of enzymatically decapped mRNA.

Authors:  A Efstratiadis; J N Vournakis; H Donis-Keller; G Chaconas; D K Dougall; F C Kafatos
Journal:  Nucleic Acids Res       Date:  1977-12       Impact factor: 16.971

4.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

5.  Molecular weight of double-stranded RNA: a re-examination of Aspergillus foetidus virus S RNA components.

Authors:  K W Buck; G Ratti
Journal:  J Gen Virol       Date:  1977-10       Impact factor: 3.891

6.  Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors:  G M Wahl; M Stern; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

7.  Rapid and efficient cosmid cloning.

Authors:  D Ish-Horowicz; J F Burke
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

8.  A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.

Authors:  G Dretzen; M Bellard; P Sassone-Corsi; P Chambon
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

9.  Functions of the two particles of tobacco rattle virus.

Authors:  H L Sänger
Journal:  J Virol       Date:  1969-03       Impact factor: 5.103

10.  Trans-complementable copy-number mutants of plasmid ColE1.

Authors:  A J Twigg; D Sherratt
Journal:  Nature       Date:  1980-01-10       Impact factor: 49.962
  10 in total
  22 in total

1.  Transfection of heteroduplexes containing uracil.guanine or thymine.guanine mispairs into plant cells.

Authors:  N M Inamdar; X Y Zhang; C L Brough; W E Gardiner; D M Bisaro; M Ehrlich
Journal:  Plant Mol Biol       Date:  1992-10       Impact factor: 4.076

2.  Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses.

Authors:  A J Howarth; J Caton; M Bossert; R M Goodman
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

3.  Molecular characterisation of subgenomic single-stranded and double-stranded DNA forms isolated from plants infected with tomato golden mosaic virus.

Authors:  S W MacDowell; R H Coutts; K W Buck
Journal:  Nucleic Acids Res       Date:  1986-10-24       Impact factor: 16.971

4.  Proliferating cell nuclear antigen transcription is repressed through an E2F consensus element and activated by geminivirus infection in mature leaves.

Authors:  E M Egelkrout; D Robertson; L Hanley-Bowdoin
Journal:  Plant Cell       Date:  2001-06       Impact factor: 11.277

5.  Geminivirus coat protein gene promoter sequences can function in Escherichia coli.

Authors:  I T Petty; R H Coutts; K W Buck
Journal:  Nucleic Acids Res       Date:  1986-06-25       Impact factor: 16.971

6.  Cloning biologically active geminivirus DNA using PCR and overlapping primers.

Authors:  V P Patel; M R Rojas; E J Paplomatas; R L Gilbertson
Journal:  Nucleic Acids Res       Date:  1993-03-11       Impact factor: 16.971

7.  Expression of functional replication protein from tomato golden mosaic virus in transgenic tobacco plants.

Authors:  L Hanley-Bowdoin; J S Elmer; S G Rogers
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

8.  DNA and RNA polymerase activities of nuclei and hypotonic extracts of nuclei isolated from tomato golden mosaic virus infected tobacco leaves.

Authors:  R H Coutts; K W Buck
Journal:  Nucleic Acids Res       Date:  1985-11-11       Impact factor: 16.971

9.  A novel motif in geminivirus replication proteins interacts with the plant retinoblastoma-related protein.

Authors:  Gerardo Arguello-Astorga; Luisa Lopez-Ochoa; Ling-Jie Kong; Beverly M Orozco; Sharon B Settlage; Linda Hanley-Bowdoin
Journal:  J Virol       Date:  2004-05       Impact factor: 5.103

10.  Demonstration of the bipartite nature of the genome of a single-stranded DNA plant virus by infection with the cloned DNA components.

Authors:  W D Hamilton; D M Bisaro; R H Coutts; K W Buck
Journal:  Nucleic Acids Res       Date:  1983-11-11       Impact factor: 16.971
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