Literature DB >> 6289745

Development of a shaker culture of Buffalo green monkey kidney cells: potential use for detection of enteroviruses.

G Goldstein, L E Guskey.   

Abstract

Buffalo green monkey kidney cells were adapted to grow as shaker cultures. Replication of environmental and clinical isolates of poliovirus, coxsackievirus, and echovirus in these cultures was analyzed by plaque assay and compared with replication in Buffalo green monkey kidney cell monolayers and HEp-2 cell shaker cultures. Dose-response tests with various concentrations of Mahoney type 1 poliovirus indicated that Buffalo green monkey kidney cell shaker cultures could detect as little as 1 PFU in an inoculum of 0.2 ml. These data suggest that Buffalo green monkey kidney cell shaker cultures can be effectively used for the detection of small quantities of enteroviruses from environmental sources.

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Year:  1982        PMID： 6289745      PMCID： PMC242012          DOI： 10.1128/aem.44.2.317-320.1982

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

1.  Application of the most probable number method for estimating concentrations of animal viruses by the tissue culture technique.

Authors:  S L CHANG; G BERG; K A BUSCH; R E STEVENSON; N A CLARKE; P W KABLER
Journal:  Virology       Date:  1958-08       Impact factor: 3.616

2.  Comparative sensitivity of various cell culture systems for isolation of viruses from wastewater and fecal samples.

Authors:  N J Schmidt; H H Ho; J L Riggs; E H Lennette
Journal:  Appl Environ Microbiol       Date:  1978-09       Impact factor: 4.792

3.  Comparative sensitivity of the BGM cell line for isolation of enteric viruses.

Authors:  N J Schmidt; H H Ho; E H Lennette
Journal:  Health Lab Sci       Date:  1976-04

4.  BGM, a continuous cell line more sensitive than primary rhesus and African green kidney cells for the recovery of viruses from water.

Authors:  D R Dahling; G Berg; D Berman
Journal:  Health Lab Sci       Date:  1974-10

5.  Estimation of low numbers of Escherichia coli bacteriophage by use of the most probable number method.

Authors:  Y Kott
Journal:  Appl Microbiol       Date:  1966-03

6.  High yield growth and purification of human parainfluenza type 3 virus and initial analysis of viral structural proteins.

Authors:  L E Guskey; G Bergtrom
Journal:  J Gen Virol       Date:  1981-05       Impact factor: 3.891

7.  Characteristics of the BGM line of cells from African green monkey kidney. Brief report.

Authors:  A L Barron; C Olshevsky; M M Cohen
Journal:  Arch Gesamte Virusforsch       Date:  1970

8.  Adaptation of BHK-21 cells to growth in shaker culture and subsequent challenge by Japanese encephalitis virus.

Authors:  L E Guskey; H M Jenkin
Journal:  Appl Microbiol       Date:  1975-09

9.  Characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the BGM/MV cell line.

Authors:  J H Menna; A R Collins; T D Flanagan
Journal:  Infect Immun       Date:  1975-01       Impact factor: 3.441
  9 in total
  1 in total

1.  Detection of poliovirus by ICC/qPCR in concentrated water samples has greater sensitivity and is less costly using BGM cells in suspension as compared to monolayers.

Authors:  Helene B Balkin; Aaron B Margolin
Journal:  Virol J       Date:  2010-10-25       Impact factor: 4.099
  1 in total

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