Literature DB >> 6289488

Perchloric acid treatment of human blood for quantitative endotoxin assay using synthetic chromogenic substrate for horseshoe crab clotting enzyme.

H Tamura, T Obayashi, K Takagi, S Tanaka, C Nakahara, T Kawai.   

Abstract

A new reliable human blood treatment was established for quantitative endotoxin assay using synthetic chromogenic substrate [Boc-Leu-Gly-Arg-rho-nitroanilide]. Addition of perchloric acid in a final concentration of 1.25% to platelet-rich plasma or serum in a 2:1 volume ratio completely eliminated nonspecific amidase activities as well as inhibitors. By this method, the recovery of added endotoxin was nearly 100%, and was almost independent of sample dilutions and anticoagulants.

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Year:  1982        PMID： 6289488     DOI： 10.1016/0049-3848(82)90277-8

Source DB:  PubMed          Journal:  Thromb Res        ISSN： 0049-3848            Impact factor:   3.944


  10 in total

1.  A study on limulus amebocyte lysate (LAL) reactive material derived from dialyzers.

Authors:  T Yoshioka; K Ikegami; K Ikemura; S Shiono; M Uenishi; H Sugimoto; T Sugimoto
Journal:  Jpn J Surg       Date:  1989-01

2.  False-positive result in Limulus test caused by Limulus amebocyte lysate-reactive material in immunoglobulin products.

Authors:  K Ikemura; K Ikegami; T Shimazu; T Yoshioka; T Sugimoto
Journal:  J Clin Microbiol       Date:  1989-09       Impact factor: 5.948

3.  Plasma endotoxin levels and functions of peripheral granulocytes in surgical patients with respiratory distress syndrome.

Authors:  T Miyata; M Torisu
Journal:  Jpn J Surg       Date:  1986-11

4.  Endogenous endotoxemia during orthotopic liver transplantation in dogs.

Authors:  T Miyata; S Todo; O Imventarza; Y Ueda; H Furukawa; T E Starzl
Journal:  Transplant Proc       Date:  1989-10       Impact factor: 1.066

5.  Non-septic endotoxemia in cirrhotic patients.

Authors:  Y Yajima; I Fukuda; M Otsuki; H Suzuki; K Mori; Y Goto
Journal:  Gastroenterol Jpn       Date:  1989-06

6.  Enhancement of dengue virus type 2 replication in mouse macrophage cultures by bacterial cell walls, peptidoglycans, and a polymer of peptidoglycan subunits.

Authors:  H Hotta; S Hotta; H Takada; S Kotani; S Tanaka; M Ohki
Journal:  Infect Immun       Date:  1983-08       Impact factor: 3.441

7.  Investigation of the renal injury caused by liver ischemia-reperfusion in rats.

Authors:  Y Kudo; T Egashira; F Takayama; Y Yamanaka; T Shimada
Journal:  Arch Toxicol       Date:  1993       Impact factor: 5.153

8.  Augmentation of endotoxin fever by recombinant human beta interferon in rabbits.

Authors:  H Kawasaki; M Moriyama; A Tanaka
Journal:  Infect Immun       Date:  1987-05       Impact factor: 3.441

Review 9.  Endotoxemia: methods of detection and clinical correlates.

Authors:  J C Hurley
Journal:  Clin Microbiol Rev       Date:  1995-04       Impact factor: 26.132

Review 10.  Outstanding Contributions of LAL Technology to Pharmaceutical and Medical Science: Review of Methods, Progress, Challenges, and Future Perspectives in Early Detection and Management of Bacterial Infections and Invasive Fungal Diseases.

Authors:  Hiroshi Tamura; Johannes Reich; Isao Nagaoka
Journal:  Biomedicines       Date:  2021-05-11
  10 in total

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