Literature DB >> 6289307

Site-directed psoralen crosslinking of DNA.

W A Saffran, M Goldenberg, C R Cantor.   

Abstract

A technique has been developed for placing site-specific crosslinks into DNA by using a psoralen derivative containing a sulfhydryl group. Plasmid pBR322 was nicked at the unique BamHI restriction site, and mercurated pyrimidines were introduced by nick-translation. Then the psoralen was specifically directed to this site in the dark through a Hg-S linkage prior to irradiation to form a crosslink. The location of interstrand cross-links was examined by electron microscopy of Pst I-cut molecules and found to be at or near the BamHI site.

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Year:  1982        PMID： 6289307      PMCID： PMC346721          DOI： 10.1073/pnas.79.15.4594

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  16 in total

1.  Inter-strand cross-linkages occurring in the photoreaction between psoralen and DNA.

Authors:  F Dall'Acqua; S Marciani; G Rodighiero
Journal:  FEBS Lett       Date:  1970-07-29       Impact factor: 4.124

2.  Electron microscopy of DNA crosslinked with trimethylpsoralen: test of the secondary structure of eukaryotic inverted repeat sequences.

Authors:  T R Cech; M L Pardue
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205

3.  Mercurated polynucleotides: new probes for hybridization and selective polymer fractionation.

Authors:  R M Dale; D C Ward
Journal:  Biochemistry       Date:  1975-06-03       Impact factor: 3.162

4.  Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis.

Authors:  R C Parker; R M Watson; J Vinograd
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

5.  Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

Authors:  J G Sutcliffe
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1979

Review 6.  Photochemistry and photobiology of psoralens.

Authors:  P S Song; K J Tapley
Journal:  Photochem Photobiol       Date:  1979-06       Impact factor: 3.421

7.  The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis.

Authors:  G S Hayward; M G Smith
Journal:  J Mol Biol       Date:  1972-02-14       Impact factor: 5.469

Review 8.  Psoralen photochemistry.

Authors:  B J Parsons
Journal:  Photochem Photobiol       Date:  1980-12       Impact factor: 3.421

9.  Photochemical production of psoralen - DNA monoadducts capable of subsequent photocrosslinking.

Authors:  P K Chatterjee; C R Cantor
Journal:  Nucleic Acids Res       Date:  1978-10       Impact factor: 16.971

10.  Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA.

Authors:  S T Isaacs; C K Shen; J E Hearst; H Rapoport
Journal:  Biochemistry       Date:  1977-03-22       Impact factor: 3.162
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  7 in total

1.  The structure of a pre-mRNA molecule in solution determined with a site directed cross-linking reagent.

Authors:  J Teare; P Wollenzien
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971

2.  Preparation and characterization of biotinylated psoralen.

Authors:  W A Saffran; J T Welsh; R M Knobler; F P Gasparro; C R Cantor; R L Edelson
Journal:  Nucleic Acids Res       Date:  1988-08-11       Impact factor: 16.971

3.  Sensitive non-radioactive dot-blot hybridization using DNA probes labelled with chelate group substituted psoralen and quantitative detection by europium ion fluorescence.

Authors:  A Oser; W K Roth; G Valet
Journal:  Nucleic Acids Res       Date:  1988-02-11       Impact factor: 16.971

4.  Specificity of site directed psoralen addition to RNA.

Authors:  J Teare; P Wollenzien
Journal:  Nucleic Acids Res       Date:  1989-05-11       Impact factor: 16.971

5.  A protected biotin containing deoxycytidine building block for solid phase synthesis of biotinylated oligonucleotides.

Authors:  U Pieles; B S Sproat; G M Lamm
Journal:  Nucleic Acids Res       Date:  1990-08-11       Impact factor: 16.971

6.  The complete pattern of mutagenesis arising from the repair of site-specific psoralen crosslinks: analysis by oligonucleotide hybridization.

Authors:  W A Saffran; C R Cantor
Journal:  Nucleic Acids Res       Date:  1984-12-21       Impact factor: 16.971

7.  Neighborhood of 16S rRNA nucleotides U788/U789 in the 30S ribosomal subunit determined by site-directed crosslinking.

Authors:  D Mundus; P Wollenzien
Journal:  RNA       Date:  1998-11       Impact factor: 4.942
  7 in total

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