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Literature DB >> 6285888

Purification of total cellular DNA from a single plant.

Abstract

A procedure is outlined for purifying DNA from a single plant. A crude organelle pellet consisting of nuclei, chromatin, chloroplasts, and mitochondria is prepared, suspended, and immediately lysed with detergents. The DNA is separated from RNA, protein, and polysaccharides by banding it in CsCl density equilibrium gradients. Ethidium bromide is included in all buffers to act as an inhibitor of DNAase activity. The DNA prepared in this manner can be digested with restriction endonucleases, separated by gel electrophoresis, and used to identify specific genes by hybridization of cloned DNA sequences.

Entities: Chemical

Mesh：

Substances：

Year: 1982 PMID： 6285888 DOI： 10.1007/bf00484419

Source DB: PubMed Journal: Biochem Genet ISSN： 0006-2928 Impact factor: 1.890

15 in total

1. Utility of ethidium bromide in the extraction from whole plants of high molecular weight maize DNA.

Authors: N Kislev; I Rubenstein
Journal: Plant Physiol Date: 1980-12 Impact factor: 8.340

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

4. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.

Authors: F R Blattner; B G Williams; A E Blechl; K Denniston-Thompson; H E Faber; L Furlong; D J Grunwald; D O Kiefer; D D Moore; J W Schumm; E L Sheldon; O Smithies
Journal: Science Date: 1977-04-08 Impact factor: 47.728

5. Nucleotide sequence divergence and functional constraint in mRNA evolution.

Authors: T Miyata; T Yasunaga; T Nishida
Journal: Proc Natl Acad Sci U S A Date: 1980-12 Impact factor: 11.205

6. Mitochondrial DNA analyses and the origin and relative age of parthenogenetic lizards (genus Cnemidophorus).

Authors: W M Brown; J W Wright
Journal: Science Date: 1979-03-23 Impact factor: 47.728

7. The use of restriction endonucleases to measure mitochondrial DNA sequence relatedness in natural populations. I. Population structure and evolution in the genus Peromyscus.

Authors: J C Avise; R A Lansman; R O Shade
Journal: Genetics Date: 1979-05 Impact factor: 4.562

8. X-ray intensifying screens greatly enhance the detection by autoradiography of the radioactive isotopes 32P and 125I.

Authors: R Swanstrom; P R Shank
Journal: Anal Biochem Date: 1978-05 Impact factor: 3.365

9. A membrane-filter technique for the detection of complementary DNA.

Authors: D T Denhardt
Journal: Biochem Biophys Res Commun Date: 1966-06-13 Impact factor: 3.575

10. Organelle DNA variation and systematic relationships in the genus Zea: Teosinte.

Authors: D H Timothy; C S Levings; D R Pring; M F Conde; J L Kermicle
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

5 in total

1. Efficient transformation with regeneration of the tropical pasture legumeStylosanthes humilis usingAgrobacterium rhizogenes and a Ti plasmid-binary vector system.

Authors: J M Manners; H Way
Journal: Plant Cell Rep Date: 1989-06 Impact factor: 4.570

5. Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules.

Authors: S V Tingey; E L Walker; G M Coruzzi
Journal: EMBO J Date: 1987-01 Impact factor: 11.598