Literature DB >> 16661592

Utility of ethidium bromide in the extraction from whole plants of high molecular weight maize DNA.

N Kislev1, I Rubenstein.   

Abstract

A procedure is reported for the isolation of high molecular weight maize DNA from whole plant tissue. Nuclei are isolated in the presence of ethidium bromide from leaf, node, and tassel or endosperm tissues and the DNA is extracted and purified. The resulting DNA has a double-strand molecular weight of about 125 kilobase pairs and a single-strand molecular weight of about 125 kilobases. The DNA is cleavable by a number of common restriction endonucleases.

Entities:  

Year:  1980        PMID： 16661592      PMCID： PMC440805          DOI： 10.1104/pp.66.6.1140

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  26 in total

1.  MOLECULAR SIZE AND CIRCULARITY OF DNA IN CELLS OF MAMMALS AND HIGHER PLANTS.

Authors:  Y HOTTA; A BASSEL
Journal:  Proc Natl Acad Sci U S A       Date:  1965-02       Impact factor: 11.205

2.  Mass isolation of pea nuclei.

Authors:  K J Tautvydas
Journal:  Plant Physiol       Date:  1971-04       Impact factor: 8.340

3.  Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis.

Authors:  R C Parker; R M Watson; J Vinograd
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

4.  Cloning of the yeast ribosomal DNA repeat unit in SstI and HindIII lambda vectors using genetic and physical size selections.

Authors:  P Philippsen; R A Kramer; R W Davis
Journal:  J Mol Biol       Date:  1978-08-15       Impact factor: 5.469

5.  Nucleotide sequence specificity of restriction endonucleases.

Authors:  H O Smith
Journal:  Science       Date:  1979-08-03       Impact factor: 47.728

6.  Separation of very large DNA molecules by gel electrophoresis.

Authors:  W L Fangman
Journal:  Nucleic Acids Res       Date:  1978-03       Impact factor: 16.971

7.  The isolation of high molecular weight DNA from whole organisms or large tissue masses.

Authors:  D E Graham
Journal:  Anal Biochem       Date:  1978-04       Impact factor: 3.365

8.  Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.

Authors:  F R Blattner; B G Williams; A E Blechl; K Denniston-Thompson; H E Faber; L Furlong; D J Grunwald; D O Kiefer; D D Moore; J W Schumm; E L Sheldon; O Smithies
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

9.  Isolation and Properties of Deoxyribonucleic Acid from Protoplasts of Cell Suspension Cultures of Ammi visnaga and Carrot (Daucus carota).

Authors:  K Ohyama; O L Gamborg; R A Miller
Journal:  Plant Physiol       Date:  1972-09       Impact factor: 8.340

10.  Dominant lethality in frog embryos after paternal treatment with triethylenemelamine: cytogenetics, morphology, and swimming capability.

Authors:  R G McKinnell; D J Picciano; J W Schaad
Journal:  Environ Mutagen       Date:  1979
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  22 in total

1.  The lectin gene family of Ricinus communis: cloning of a functional ricin gene and three lectin pseudogenes.

Authors:  J W Tregear; L M Roberts
Journal:  Plant Mol Biol       Date:  1992-02       Impact factor: 4.076

2.  Regulation of a stylar transmitting tissue-specific gene in wild-type and transgenic tomato and tobacco.

Authors:  K A Budelier; A G Smith; C S Gasser
Journal:  Mol Gen Genet       Date:  1990-11

3.  ADPglucose Pyrophosphorylase Is Encoded by Different mRNA Transcripts in Leaf and Endosperm of Cereals.

Authors:  H B Krishnan; C D Reeves; T W Okita
Journal:  Plant Physiol       Date:  1986-06       Impact factor: 8.340

4.  Diversity of Vicia faba circular mtDNA in whole plants and suspension cultures.

Authors:  V I Negruk; G I Eisner; T D Redichkina; N N Dumanskaya; D I Cherny; A A Alexandrov; M F Shemyakin; R G Butenko
Journal:  Theor Appl Genet       Date:  1986-07       Impact factor: 5.699

5.  Preparation of high molecular weight plant DNA and its use for artificial chromosome construction.

Authors:  A Balestrazzi; G Bernacchia; R Cella; L Ferretti; S Sora
Journal:  Plant Cell Rep       Date:  1991-09       Impact factor: 4.570

6.  Recombination within the inverted repeat sequences of the Chlamydomonas reinhardii chloroplast genome produces two orientation isomers.

Authors:  J Aldrich; B Cherney; E Merlin; C Williams; L Mets
Journal:  Curr Genet       Date:  1985-03       Impact factor: 3.886

7.  Analysis of the genes encoding the largest subunit of RNA polymerase II in Arabidopsis and soybean.

Authors:  M A Dietrich; J P Prenger; T J Guilfoyle
Journal:  Plant Mol Biol       Date:  1990-08       Impact factor: 4.076

8.  Nucleotide sequence and molecular characterization of a maize mitochondrial plasmid-like DNA.

Authors:  A G Smith; D R Pring
Journal:  Curr Genet       Date:  1987       Impact factor: 3.886

9.  Compositional compartmentalization and compositional patterns in the nuclear genomes of plants.

Authors:  J Salinas; G Matassi; L M Montero; G Bernardi
Journal:  Nucleic Acids Res       Date:  1988-05-25       Impact factor: 16.971

10.  Aleurones from a Barley with Low [alpha]-Amylase Activity Become Highly Responsive to Gibberellin When Detached from the Starchy Endosperm.

Authors:  R. W. Skadsen
Journal:  Plant Physiol       Date:  1993-05       Impact factor: 8.340
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