| Literature DB >> 6285149 |
W Boidol, M Simonis, M Töpert, G Siewert.
Abstract
Restriction maps of several recombinant plasmids representing a section of the E. coli K12 chromosome 35,000 bp in size with the genes phoA, proC and phoB were prepared. The orientation of phoA and the exact position of its N-terminal end on this map were determined by identifying a subfragment which carried the phoA promoter and by determining the nucleotide sequence of a 160 bp portion of this subfragment comprising the codons for the N-terminal end of pre-alkaline phosphatase. From this DNA sequence the leader sequence of alkaline phosphatase which consists of 21 amino acids was derived.Entities:
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Year: 1982 PMID: 6285149 DOI: 10.1007/bf00334150
Source DB: PubMed Journal: Mol Gen Genet ISSN: 0026-8925