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Literature DB >> 6283471

Cloning and characterization of cDNA copies of the 7S RNAs of HeLa cells.

Abstract

We have cloned cDNA copies of in vitro adenylated 7S RNA of HeLa cells. The most representative clones in the library contain DNA fragments copied from the 7SL and 7SK small RNAs. The two classes of recombinants share no homology. The 7SL RNA contains at the 5' end of the molecule sequences homologous to the Alu sequence family. Hybridization to human genomic DNA shows that the 7SL and 7SK clones are homologous to two different families of repetitive sequences.

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Year: 1982 PMID： 6283471 PMCID： PMC320604 DOI： 10.1093/nar/10.7.2209

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

28 in total

1. Clustering of the DNA sequences complementary to repetitive nuclear RNA of HeLa cells.

Authors: M Melli
Journal: J Mol Biol Date: 1975-03-25 Impact factor: 5.469

2. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors: T Maniatis; A Jeffrey; H van deSande
Journal: Biochemistry Date: 1975-08-26 Impact factor: 3.162

3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

4. Small RNA species of the HeLa cell: metabolism and subcellular localization.

Authors: G Zieve; S Penman
Journal: Cell Date: 1976-05 Impact factor: 41.582

5. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors: W D Benton; R W Davis
Journal: Science Date: 1977-04-08 Impact factor: 47.728

6. Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.

Authors: A E Sippel
Journal: Eur J Biochem Date: 1973-08-01

7. Comparison of oligonucleotides produced by RNase T1 digestion of 7 S RNA from avian and murine oncornaviruses and from uninfected cells.

Authors: E Erikson; R L Erikson; B Henry; N R Pace
Journal: Virology Date: 1973-05 Impact factor: 3.616

8. The 7S RNA common to oncornaviruses and normal cells is associated with polyribosomes.

Authors: T A Walker; N R Pace; R L Erikson; E Erikson; F Behr
Journal: Proc Natl Acad Sci U S A Date: 1974-09 Impact factor: 11.205

9. On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes.

Authors: R P Perry; J La Torre; D E Kelley; J R Greenberg
Journal: Biochim Biophys Acta Date: 1972-03-14

10. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.

Authors: M P Wickens; G N Buell; R T Schimke
Journal: J Biol Chem Date: 1978-04-10 Impact factor: 5.157

12 in total

Cloning and characterization of cDNA copies of the 7S RNAs of HeLa cells.

1. Clustering of the DNA sequences complementary to repetitive nuclear RNA of HeLa cells.

2. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

4. Small RNA species of the HeLa cell: metabolism and subcellular localization.

5. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

6. Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.

7. Comparison of oligonucleotides produced by RNase T1 digestion of 7 S RNA from avian and murine oncornaviruses and from uninfected cells.

8. The 7S RNA common to oncornaviruses and normal cells is associated with polyribosomes.

9. On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes.

10. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.

1. Characterization of the 7S RNA and its gene from halobacteria.

2. Linkage of a 7S RNA sequence and kappa light chain genes in the mouse.

3. Localization of small RNAs hybridizable to a B2 clone in the nuclear fraction of mouse cell lines.

4. Cloning and characterisation of the abundant cytoplasmic 7S RNA from mouse cells.

5. Unusual class of Alu sequences containing a potential Z-DNA segment.

6. Observations on the structure of two human 7SK pseudogenes and on homologous transcripts in vertebrate species.

7. Characterization of the signal recognition particle (SRP) RNA population of tomato (Lycopersicon esculentum).

8. Ribonucleoprotein particles of quiescent maize embryonic axes.

9. Human autoantibodies reactive with the signal-recognition particle.

10. Human genes and pseudogenes for the 7SL RNA component of signal recognition particle.