Literature DB >> 6283105

Evidence the pp60src, the product of the Rous sarcoma virus src gene, undergoes autophosphorylation.

A F Purchio.   

Abstract

pp60src, the product of the rous sarcoma virus src gene, was purified greater than 100,000-fold by a combination of ion-exchange and immunoaffinity chromatography. Incubation of pp60src purified in this fashion with [32P-gamma]ATP resulted in a single 32P-labeled protein when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Staining of these gels with silver nitrate showed a predominant 60,000-dalton polypeptide which comigrated with the protein labeled with 32P in vitro. Partial digestion of this protein with V8 protease after in vitro iodination indicated that it was pp60src. These results suggest that pp60src is able to autophosphorylate.

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Year:  1982        PMID： 6283105      PMCID： PMC256720     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  19 in total

1.  Identification of a transformation-specific antigen induced by an avian sarcoma virus.

Authors:  J S Brugge; R L Erikson
Journal:  Nature       Date:  1977-09-22       Impact factor: 49.962

2.  Protein kinase activity associated with the avian sarcoma virus src gene product.

Authors:  M S Collett; R L Erikson
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

3.  Identification of a polypeptide encoded by the avian sarcoma virus src gene.

Authors:  A F Purchio; E Erikson; J S Brugge; R L Erikson
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

Review 4.  Phosphorylation-dephosphorylation of enzymes.

Authors:  E G Krebs; J A Beavo
Journal:  Annu Rev Biochem       Date:  1979       Impact factor: 23.643

5.  Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation.

Authors:  M S Collett; E Erikson; R L Erikson
Journal:  J Virol       Date:  1979-02       Impact factor: 5.103

6.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors:  D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

7.  An adenosine 3',5'-monophosphate-dependant protein kinase from rabbit skeletal muscle.

Authors:  D A Walsh; J P Perkins; E G Krebs
Journal:  J Biol Chem       Date:  1968-07-10       Impact factor: 5.157

8.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

9.  Characterization of a normal avian cell protein related to the avian sarcoma virus transforming gene product.

Authors:  M S Collett; J S Brugge; R L Erikson
Journal:  Cell       Date:  1978-12       Impact factor: 41.582

10.  The enzymatic preparation of [alpha-32P]ATP, [alpha-32P]GTP, [32P]cAMP, and [32P]cGMP, and their use in the assay of adenylate and guanylate cyclases and cyclic nucleotide phosphodiesterases.

Authors:  R A Johnson; T F Walseth
Journal:  Adv Cyclic Nucleotide Res       Date:  1979
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  18 in total

1.  Expression of v-src in embryonic neural retina alters cell adhesion, inhibits histogenesis, and prevents induction of glutamine synthetase.

Authors:  L Vardimon; L E Fox; R Cohen-Kupiec; L Degenstein; A A Moscona
Journal:  Mol Cell Biol       Date:  1991-10       Impact factor: 4.272

2.  Inhibition of the tyrosine kinase activity of v-src, v-fgr, and v-yes gene products by a monoclonal antibody which binds both amino and carboxy peptide fragments of pp60v-src.

Authors:  D J McCarley; J T Parsons; D C Benjamin; S J Parsons
Journal:  J Virol       Date:  1987-06       Impact factor: 5.103

3.  Low level of cellular protein phosphorylation by nontransforming overproduced p60c-src.

Authors:  H Iba; F R Cross; E A Garber; H Hanafusa
Journal:  Mol Cell Biol       Date:  1985-05       Impact factor: 4.272

4.  Deletions and insertions within an amino-terminal domain of pp60v-src inactivate transformation and modulate membrane stability.

Authors:  H C Wang; J T Parsons
Journal:  J Virol       Date:  1989-01       Impact factor: 5.103

5.  Autophosphorylation activates c-Src kinase through global structural rearrangements.

Authors:  Edgar E Boczek; Qi Luo; Marco Dehling; Michael Röpke; Sophie L Mader; Andreas Seidl; Ville R I Kaila; Johannes Buchner
Journal:  J Biol Chem       Date:  2019-07-22       Impact factor: 5.157

6.  Characterization of pp60src phosphorylation in vitro in Rous sarcoma virus-transformed cell membranes.

Authors:  M D Resh; R L Erikson
Journal:  Mol Cell Biol       Date:  1985-05       Impact factor: 4.272

7.  Purification of the Rous sarcoma virus src kinase by casein-agarose and tyrosine-agarose affinity chromatography.

Authors:  Y Fukami; F Lipmann
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

8.  Phosphorylation of the transforming protein of Rous sarcoma virus: direct demonstration of phosphorylation of serine 17 and identification of an additional site of tyrosine phosphorylation in p60v-src of Prague Rous sarcoma virus.

Authors:  T Patschinsky; T Hunter; B M Sefton
Journal:  J Virol       Date:  1986-07       Impact factor: 5.103

Review 9.  Autophosphorylation: a salient feature of protein kinases.

Authors:  J A Smith; S H Francis; J D Corbin
Journal:  Mol Cell Biochem       Date:  1993-11       Impact factor: 3.396

10.  Functional domains of the pp60v-src protein as revealed by analysis of temperature-sensitive Rous sarcoma virus mutants.

Authors:  A W Stoker; P J Enrietto; J A Wyke
Journal:  Mol Cell Biol       Date:  1984-08       Impact factor: 4.272
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