| Literature DB >> 6282713 |
Abstract
The construction of a plasmid vector which facilitates the cloning and recovery of blunt-ended DNA fragments is described. This plasmid, called pHP34, differs from pBR322 by a 10-bp insertion which introduces a unique SmaI site immediately flanked by two EcoRI sites. Blunt-ended DNA fragments cloned in the SmaI site can be recovered by digestion with EcoRI. Small cloned fragments can be chemically sequenced using a strategy which does not require their purification. The use of a plasmid related to pHP34 for in vitro mutagenesis by the insertion of a DNA linker fragment conferring an antibiotic resistance is also discussed.Entities:
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Year: 1982 PMID： 6282713 DOI： 10.1016/0378-1119(82)90072-5
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688