Literature DB >> 6282684

Cloning of bacteriophage T5 DNA fragments. III. Expression in Escherichia coli mini-cells.

F Brunel, J Davison, V Ha-Thi, J Reeve.   

Abstract

Use has been made of the mini-cell system to study polypeptide synthesis from cloned EcoRI, HindIII and PstI fragments of T5 DNA. The correlation of certain gene products with known genes has been established, as well as the physical mapping of genes not yet identified genetically. In some cases, it has been possible to demonstrate the presence of T5 promoters on the cloned DNA fragments. The design of experiments to avoid certain artifacts inherent in the use of the mini-cell system is discussed.

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Year:  1981        PMID: 6282684     DOI: 10.1016/0378-1119(81)90067-6

Source DB:  PubMed          Journal:  Gene        ISSN: 0378-1119            Impact factor:   3.688


  4 in total

1.  Direction of transcription in bacteriophage T5 first-step transfer DNA.

Authors:  J Davison; D Lafontaine
Journal:  J Virol       Date:  1984-05       Impact factor: 5.103

2.  Transcription regulatory elements in the late region of bacteriophage T5 DNA.

Authors:  F Brunel; V H Thi; M F Pilaete; J Davison
Journal:  Nucleic Acids Res       Date:  1983-11-11       Impact factor: 16.971

3.  Cloning and expression of a Bacillus coagulans amylase gene in Escherichia coli.

Authors:  P Cornelis; C Digneffe; K Willemot
Journal:  Mol Gen Genet       Date:  1982

4.  Cloning and expression of the Staphylococcus aureus protein A gene in Escherichia coli.

Authors:  C J Duggleby; S A Jones
Journal:  Nucleic Acids Res       Date:  1983-05-25       Impact factor: 16.971

  4 in total

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