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Literature DB >> 6278446

In vitro transcription of a cloned mouse ribosomal RNA gene.

Y Mishima, O Yamamoto, R Kominami, M Muramatsu.

Abstract

An in vitro transcription system which utilizes cloned mouse ribosomal RNA gene (rDNA) fragments and a mouse cell extract has been developed. RNA polymerases I is apparently responsible for this transcription as evidenced by the complete resistance to a high concentration (200 micrograms/ml) of alpha-amanitin. Run-off products obtained with three different truncated rDNA fragments indicated that RNA was transcribed from a unique site of rDNA. The S1 nuclease protection mapping of the in vitro product and of in vivo 45S RNA confirmed this site, indicating that, in this in vitro system, transcription of rDNA started from the same site as in vivo. This site is located at several hundred nucleotides upstream from the putative initiation site reported by us (1) and by others (2). Some sequence homology surrounding this region was noted among mouse, Xenopus laevis and Drosophila melanogaster. The data also suggest that some processing of the primary transcript occurs in this in vitro system.

Entities: Chemical Species

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Year: 1981 PMID： 6278446 PMCID： PMC327640 DOI： 10.1093/nar/9.24.6773

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

26 in total

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Authors: T C Currier; E W Nester
Journal: Anal Biochem Date: 1976-12 Impact factor: 3.365

2. Heterogeneity of 5' -termini of nucleolar 45S, 32S and 28S RNA in mouse hepatoma.

Authors: R Kominami; M Muramatsu
Journal: Nucleic Acids Res Date: 1977-01 Impact factor: 16.971

3. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors: A J Berk; P A Sharp
Journal: Cell Date: 1977-11 Impact factor: 41.582

4. Synthesis of ribosomal 5S RNA by isolated nuclei from HeLa cells in vitro.

Authors: M Yamamoto; K H Seifart
Journal: Biochemistry Date: 1977-07-12 Impact factor: 3.162

5. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

6. A method for the recovery of DNA from agarose gels.

Authors: H F Tabak; R A Flavell
Journal: Nucleic Acids Res Date: 1978-07 Impact factor: 16.971

7. The mechanism of decrease in nucleolar RNA synthesis by protein synthesis inhibition.

Authors: Y Mishima; T Matsui; M Muramatsu
Journal: J Biochem Date: 1979-03 Impact factor: 3.387

8. The ribonuclease activity of crystallized pancreatic deoxyribonuclease.

Authors: S B Zimmerman; D Sandeen
Journal: Anal Biochem Date: 1966-02 Impact factor: 3.365

9. Adenovirus DNA-directed transcription of 5.5S RNA in vitro.

Authors: G J Wu
Journal: Proc Natl Acad Sci U S A Date: 1978-05 Impact factor: 11.205

10. Selective and accurate transcription of the Xenopus laevis 5S RNA genes in isolated chromatin by purified RNA polymerase III.

Authors: C S Parker; R G Roeder
Journal: Proc Natl Acad Sci U S A Date: 1977-01 Impact factor: 11.205

33 in total

1. Transcription of the rRNA gene cluster in Aspergillus nidulanss.

Authors: E Bartnik; P Borsuk
Journal: Curr Genet Date: 1983-04 Impact factor: 3.886

2. Transcription of herpes simplex virus tk sequences under the control of wild-type and mutant human RNA polymerase I promoters.

Authors: S T Smale; R Tjian
Journal: Mol Cell Biol Date: 1985-02 Impact factor: 4.272

3. Presence of a limited number of essential nucleotides in the promoter region of mouse ribosomal RNA gene.

Authors: T Kishimoto; M Nagamine; T Sasaki; N Takakusa; T Miwa; R Kominami; M Muramatsu
Journal: Nucleic Acids Res Date: 1985-05-24 Impact factor: 16.971

4. Transfection of mouse ribosomal DNA into rat cells: faithful transcription and processing.

Authors: V B Vance; E A Thompson; L H Bowman
Journal: Nucleic Acids Res Date: 1985-10-25 Impact factor: 16.971

Review 5. Transcription of eukaryotic ribosomal RNA gene.

Authors: S T Jacob
Journal: Mol Cell Biochem Date: 1986-04 Impact factor: 3.396

6. Factors and nucleotide sequences that direct ribosomal DNA transcription and their relationship to the stable transcription complex.

Authors: J Tower; V C Culotta; B Sollner-Webb
Journal: Mol Cell Biol Date: 1986-10 Impact factor: 4.272

7. Human ribosomal RNA gene: nucleotide sequence of the transcription initiation region and comparison of three mammalian genes.

Authors: I Financsek; K Mizumoto; Y Mishima; M Muramatsu
Journal: Proc Natl Acad Sci U S A Date: 1982-05 Impact factor: 11.205