Literature DB >> 6278256

Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn10.

R Morona, P Reeves.   

Abstract

We have cloned the tolC gene of E. coli K-12 into pSF2124 by using transposon Tn10 as the marker to first isolate the relevant DNA fragment. The gene is on a 10.5 kb EcoRI fragment, and Tn5 insertion mutagenesis locates the gene near one end of this EcoRI fragment. An EcoRI-PstI fragment has been subcloned into pBR322 to facilitate further analysis of the gene.

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Year:  1981        PMID： 6278256     DOI： 10.1007/bf00352517

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  19 in total

1.  The genome of Bacillus subtilis phage SPP1: the arrangement of restriction endonuclease generated fragments.

Authors:  S W Ratcliff; J Luh; A T Ganesan; B Behrens; R Thompson; M A Montenegro; G Morelli; T A Trautner
Journal:  Mol Gen Genet       Date:  1979-01-10

2.  Plasmid identification using specific endonucleases.

Authors:  R Thompson; S G Hughes; P Broda
Journal:  Mol Gen Genet       Date:  1974

3.  Genetics and physiology of colicin-tolerant mutants of Escherichia coli.

Authors:  R Nagel de Zwaig; S E Luria
Journal:  J Bacteriol       Date:  1967-10       Impact factor: 3.490

4.  Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product.

Authors:  N E Murray; S A Bruce; K Murray
Journal:  J Mol Biol       Date:  1979-08-15       Impact factor: 5.469

5.  The tolC locus in Escherichia coli K12.

Authors:  E N Whitney
Journal:  Genetics       Date:  1971-01       Impact factor: 4.562

6.  A simple method for the preparation of large quantities of pure plasmid DNA.

Authors:  G O Humphreys; G A Willshaw; E S Anderson
Journal:  Biochim Biophys Acta       Date:  1975-04-02

Review 7.  Linkage map of Escherichia coli K-12, edition 6.

Authors:  B J Bachmann; K B Low
Journal:  Microbiol Rev       Date:  1980-03

8.  Homology of the gene coding for outer membrane lipoprotein within various Gram-negative bacteria.

Authors:  K Nakamura; R M Pirtle; M Inouye
Journal:  J Bacteriol       Date:  1979-01       Impact factor: 3.490

9.  Amplification of the respiratory NADH dehydrogenase of Escherichia coli by gene cloning.

Authors:  I G Young; A Jaworowski; M I Poulis
Journal:  Gene       Date:  1978-09       Impact factor: 3.688

10.  The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.

Authors:  M So; R Gill; S Falkow
Journal:  Mol Gen Genet       Date:  1975-12-30
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  14 in total

Review 1.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors:  M K Berlyn
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

2.  Escherichia coli K-12 outer membrane protein (OmpA) as a bacteriophage receptor: analysis of mutant genes expressing altered proteins.

Authors:  R Morona; M Klose; U Henning
Journal:  J Bacteriol       Date:  1984-08       Impact factor: 3.490

3.  Identification and characterization of the TolC protein, an outer membrane protein from Escherichia coli.

Authors:  R Morona; P A Manning; P Reeves
Journal:  J Bacteriol       Date:  1983-02       Impact factor: 3.490

Review 4.  Linkage map of Escherichia coli K-12, edition 7.

Authors:  B J Bachmann
Journal:  Microbiol Rev       Date:  1983-06

5.  Primary structure of the tolC gene that codes for an outer membrane protein of Escherichia coli K12.

Authors:  J Hackett; P Reeves
Journal:  Nucleic Acids Res       Date:  1983-09-24       Impact factor: 16.971

6.  Genetic assessment of the role of AcrB β-hairpins in the assembly of the TolC-AcrAB multidrug efflux pump of Escherichia coli.

Authors:  Jon W Weeks; Vassiliy N Bavro; Rajeev Misra
Journal:  Mol Microbiol       Date:  2014-01-21       Impact factor: 3.501

7.  Molecular cloning, characterization, and nucleotide sequence of the rfc gene, which encodes an O-antigen polymerase of Salmonella typhimurium.

Authors:  L V Collins; J Hackett
Journal:  J Bacteriol       Date:  1991-04       Impact factor: 3.490

8.  A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.

Authors:  M Singer; T A Baker; G Schnitzler; S M Deischel; M Goel; W Dove; K J Jaacks; A D Grossman; J W Erickson; C A Gross
Journal:  Microbiol Rev       Date:  1989-03

9.  New locus (ttr) in Escherichia coli K-12 affecting sensitivity to bacteriophage T2 and growth on oleate as the sole carbon source.

Authors:  R Morona; U Henning
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

10.  Cellular localization and export of the soluble haemolysin of Vibrio cholerae El Tor.

Authors:  A Mercurio; P A Manning
Journal:  Mol Gen Genet       Date:  1985
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