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Literature DB >> 6276692

P1 transduction map spanning the replication terminus of Escherichia coli K12.

Abstract

The region of the E. coli chromosome that contains the replication terminus has not previously been spanned by P1 cotransduction. We have used Tn5, Tn9 and Tn10 transposons inserted in this region as genetic markers, and have constructed a genetic map that extends from fnr (min 29.3) to manA (min 35.7). The relevant transposons that have been mapped in this region and which are described in this report are trg-1::Tn5 (min 31.1), zdc-235::Tn10 (min 32.3), zdd-230::Tn9 (min 33.3), and zde-234::Tn10 (min 34.2). The size of this region as determined by P1 cotransduction is very similar to previous estimates obtained by bacterial conjugation.

Entities: Gene Species

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Year: 1981 PMID： 6276692 DOI： 10.1007/bf00272906

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

19 in total

5. marA locus causes decreased expression of OmpF porin in multiple-antibiotic-resistant (Mar) mutants of Escherichia coli.

Authors: S P Cohen; L M McMurry; S B Levy
Journal: J Bacteriol Date: 1988-12 Impact factor: 3.490

P1 transduction map spanning the replication terminus of Escherichia coli K12.

1. Chromosome replication in Escherichia coli is inhibited in the terminus region near the rac locus.

2. Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.

3. Evidence for a fixed termination site of chromosome replication in Escherichia coli K12.

4. Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12.

5. Regulation of Tn5 transposition in Salmonella typhimurium.

6. Transductional mapping of ksgB and a new Tn5-induced kasugamycin resistance gene, ksgD, in Escherichia coli K-12.

Review 7. Linkage map of Escherichia coli K-12, edition 6.

8. Fine-structure mapping and complementation analysis of the Escherichia coli cysB gene.

9. Mutants of Escherichia coli K12 unable to use fumarate as an anaerobic electron acceptor.

10. Terminus region of the chromosome in Escherichia coli inhibits replication forks.

1. Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome.

2. Termination sites T1 and T2 from the Escherichia coli chromosome inhibit DNA replication in ColE1-derived plasmids.

3. Activation of oxidative stress genes by mutations at the soxQ/cfxB/marA locus of Escherichia coli.

Review 4. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

5. marA locus causes decreased expression of OmpF porin in multiple-antibiotic-resistant (Mar) mutants of Escherichia coli.

6. Cloning of the contiguous 165-kilobase-pair region around the terminus of Escherichia coli K-12 DNA replication.

7. Deletion of the terminus region (340 kilobase pairs of DNA) from the chromosome of Escherichia coli.

8. Zygotic induction of the rac locus can cause cell death in E. coli.

Review 9. Linkage map of Escherichia coli K-12, edition 7.

10. Genetic mapping of the minB locus in Escherichia coli K-12.